杜仲绿原酸对高脂模型小鼠降血脂作用研究
详细信息Effect of chlorogenic acid extracted from Eucommia Ulmoides Oliv on hyperlipemia of mice induced by high fat diet
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摘要: 旨在开展杜仲绿原酸对高脂高胆固醇诱导的高血脂模型小鼠脂质代谢的影响研究,以昆明小鼠为实验动物,随机分成5组:阴性对照组,模型对照组,以及低剂量（25mg/kgBW）、中剂量（50mg/kgBW）、高剂量（100mg/kgBW）杜仲绿原酸组,每组10只。后4组喂以高脂饲料,将小鼠灌胃杜仲绿原酸4周,分别测定各组小鼠血清和肝脏脂质水平及血清和肝脏的抗氧化酶活性和脂质过氧化产物MDA含量。研究发现:杜仲绿原酸显著降低小鼠血清TC、TG、LDL-C水平、动脉硬化指数和冠心指数,肝脏TC、TG含量亦有显著降低,血清和肝脏中MDA生成下降,抗氧化酶活性增加。研究表明:在高脂膳食条件下,杜仲绿原酸能够有效地降低血脂和肝脏脂质的积累,提高血清和肝脏抗氧化水平,具有明显的调节脂质代谢作用,其中以中剂量降血脂效果相对最佳。Abstract: To investigate the effect of chlorogenic acid extracted from Eucommia Ulmoides Oliv on lipid metabolism of hyperlipemia mice, fifty Kunming mice were randomly divided into 5 groups:negative control group, model control group, low dose (25mg/kg BW) , medium dose (50mg/kg BW) , and high dose (100mg/kg BW) groups of chlorogenic acid.The latter 4 groups were fed with high fat feed.Chlorogenic acid was given by gavage.Serum and liver lipid metabolic indices and their antioxidant enzymes activities and peroxidation product were measured.When chlorogenic acid was fed, the contents of serum TC, TG, LDL-C levels and liver TC, TG were significantly lower.Furthermore, MDA contents in serum and liver were decreased, and activities of antioxidant enzymes were increased.AI and R-CHR were also lower than that of the model group.These results indicated that chlorogenic acid from Eucommia Ulmoides Oliv could effectively reduce the blood and liver lipid accumulation and regulate lipid metabolism by improving their antioxidant activities.Among them the group at the dose of 50mg/kg BW showed best effect.
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