响应面法优化仿栗籽蛋白提取工艺
详细信息Optimization of the extraction technology of protein from sloanea hemsleyana seed by response surface methodology
-
摘要: 研究了仿栗籽蛋白的碱溶酸沉法提取工艺。在单因素实验的基础上,选定提取温度、pH、提取时间、液料比四个因素的三个水平进行中心组合实验,建立了仿栗籽蛋白提取率的二次回归方程,通过响应面分析以及岭嵴分析得到了优化组合条件。实验结果表明,当提取工艺条件为pH9.6、温度42℃、时间138min、料液比1∶25(g/mL)时,仿栗籽蛋白提取率为64.97%。Abstract: The extraction of protein from the sloanea hemsleyana seed by alkaline extraction and acid precipitation was studied. According to the single-factor experiments, three levels of the factors (extraction temperature, extraction time, pH and material/liquid ratio) were selected for the central composite design to establish a quadric regression equation for describing the yield of sloanea hemsleyana seed protein. By response surface analysis, the optimum extraction conditions were obtained as follows:extraction temperature of 42℃, time of 138min, pH9.6 and material/liquid ratio of 1∶25 (g/mL) , under which the highest yield of sloanea hemsleyana seed protein reached 64.97%.
-
[1] 李昌珠, 张良波, 向祖恒, 等.仿栗生物学特性及果实油脂含量分析[J].湖南林业科技, 2008, 35 (2) :4-8. [2] 胡欣欣, 麻成金, 黄群, 等.超声波辅助萃取仿栗籽油的研究[J].江苏食品与发酵, 2008 (1) :11-14. [3] 麻成金, 吴竹青, 傅伟昌, 等.响应面法优化仿栗籽油超临界萃取工艺[J].食品科学, 2010, 31 (18) :196-202. [4] 戴宇翔, 钱志娟, 曾晓雄, 等.菜籽蛋白提取工艺的优化[J].食品工业科技, 2011, 32 (3) :304-306. [5] 逯与运, 麻成金, 黄群, 等.响应面优化超声波辅助水酶法提取蜡梅籽蛋白[J].中国食物与营养, 2010 (9) :59-63. [6] 齐宝坤, 江连洲, 李杨, 等.响应面优化超声波辅助水酶法提取花生蛋白工艺[J].食品工业科技, 2011, 32 (11) :253-256. [7] Sayan Gupta, Manohar C S.An improved response surface method for the determination of failure probability and importance measures[J].Structural Safety, 2004 (26) :123-132.
[8] 邓红, 田芸芸, 田子卿, 等.响应曲面法优化文冠果种仁蛋白的碱溶酸沉提取工艺[J].食品工业科技, 2010, 31 (8) :197-200. [9] 许英一, 李红, 杨伟光, 等.碱提酸沉法提取紫花苜蓿叶蛋白初探[J].食品工业科技, 2011, 32 (6) :264-266. [10] Jebrail MJ, Wheeler AR.Digital microfluidic method for protein extraction by precipitation[J].Analytical Chemistry, 2009, 81 (1) :330-335.
[11] Bernardi Ra, Negri A, Ronchi S, et al.Isolation of the epithiospecifier protein from oil-rape (Brassica napus ssp.oleifera) seed and its characterization[J].FEBS Letters, 2000, 467 (2-3) :296-298.
[12] 黄群, 麻成金, 孙术国, 等.超声波辅助提取蚕豆蛋白及其功能特性研究[J].食品与发酵工业, 2009, 35 (8) :179-182. [13] 丁丹华, 彭光华, 夏辉, 等.油茶籽粕蛋白提取工艺研究[J].食品科学, 2010, 31 (8) :102-105. [14] Li Jing-en, Nie Shao-ping, Yang Chao, et al.Extraction optimization, characterization and bioactivity of crude polysaccharides from Herba Moslae[J].Carbohydrate Polymers, 2011, 38 (3) :1201-1206.
[15] 李新华, 富艳鑫, 郑煜焱.米糠蛋白提取工艺条件的优化[J].食品科学, 2010, 31 (22) :251-254. [16] 王振宇, 周芳, 赵鑫.响应面分析法优化超声波提取大果沙棘总黄酮工艺[J].中国食品学报, 2007, 7 (6) :44-51. [17] 朱建飞, 吴谋成, 冯睿, 等.菜籽粕中水溶性多糖提取工艺优化[J].农业工程学报, 2006, 22 (12) :251-254.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
