牛蒡根总黄酮抗氧化活性研究
详细信息Study on antioxidant activity of total flavonoids of burdock root
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摘要: 以牛蒡根为材料,采用常规方法测定牛蒡根总黄酮的DPPH自由基清除能力、羟基自由基清除能力、总抗氧化能力、还原力、金属螯合能力、抗脂质过氧化能力和对DNA氧化损失的保护作用,同时测定了牛蒡根总黄酮的含量。结果表明,牛蒡根总黄酮的自由基清除能力、总抗氧化能力、金属螯合能力和还原力随浓度的增加而增强,且呈剂量依赖性相关,DPPH自由基清除能力的半数抑制浓度(IC50)为15.7mg/mL;对大鼠肝脂质过氧化产生的丙二醛(MDA)有较好的清除效果,IC50为34μg/mL;对K562细胞DNA氧化损伤具有保护作用。由此说明,牛蒡根为一种很好的天然抗氧化剂。Abstract: With burdock root as materials, determining total flavonoids of burdock root for their potential antioxidant activity.Therefore, the antioxidant activity assays included DPPH free-radical scavenging capacity, hydroxyl radical scavenging activity, total antioxidant capacity, reducing power, Fe2+ chelating ability, the inhibitory activity toward lipid peroxidation and protective effect on DNA oxidative damage.Total flavonoid contents were also determined.Total flavonoids of burdock root exhibited strong DPPH free-radical scavenging (IC50=15.7mg/mL) activity, hydroxyl radical scavenging activity, metal chelating ability, total antioxidant capacity, reducing power and inhibitory activity toward lipid peroxidation (34μg/mL) .Meanwhile, total flavonoids of burdock root had also a protective effect on K562 cell DNA oxidative damage.Furthermore, these results demonstrated that potential antioxidant activities of burdock root, providing scientific support for the empirical use of burdock root as an antioxidant for diseases caused by reactive oxygen species.
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