牛肺脏中肝素提取工艺优化
详细信息Optimization of heparin extraction process from bovine lung
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摘要: 本研究以牛肺脏为原料,采用盐解-树脂吸附及蛋白质沉淀除去杂质的方法提取和分离肝素。在单因素实验基础上,选取料液比、盐浓度、树脂用量,利用旋转正交组合设计原理和响应面分析法进行工艺优化。结果表明,最佳提取工艺为料液比1:15（g:mL）,盐浓度5%,树脂量7%,此条件下肝素得率理论值为362.02mg/kg,验证实验肝素得率为356.65mg/kg。盐解-树脂吸附方法可行可靠,重复性好,提取完全,更适合工厂化生产,且为秦宝雪花牛肺脏中肝素的开发利用提供理论依据。Abstract: The bovine lungs as the research material, extracted and separated heparin with the salt solution-resin adsorption and protein to remove impurities precipitation method.Three extraction parameters including material liquid ratio, salt concentration and resin content were optimized using rotating orthogonal combination design principle and the response surface method for process optimization.The results indicated that the best extraction process were material liquid ratio for 1:15 (g:mL) , salt concentration at 5% and resin content of 7%.According to these conditions, the yield of heparin was 362.02mg/kg, and under the verificative test condition the practical values was 356.65mg/kg.Salt solution-resin adsorption method was feasible and reliable, repeatability, extraction completely, more suitable for industrial production and providing the theory for the development and utilization of lungs heparin of QinBao cattle.
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