淀粉废水发酵产细菌纤维素发酵条件的优化
详细信息Optimization of culture conditions of producing bacterial cellulose utilizing starch wastewater
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摘要: 以玉米淀粉废水添加葡萄糖20g/L,玉米浆40g/L,乙醇150mL/L为发酵基质,采用单因素和正交实验设计对葡糖醋杆菌(Gluconacetobacter xylinus)发酵产细菌纤维素条件进行优化。结果表明,最佳发酵条件为:装液量80mL/250mL,pH4.0,接种量9%(V/V),温度28℃;在此条件下得到细菌纤维素产量为4.41g/L。采用傅立叶转换红外光谱FTIR验证产物为细菌纤维素,并由SEM扫描电镜观察纤维素膜表面形貌。Abstract: The culture conditions of Gluconacetobacter xylinus producing bacterial cellulose utilizing corn starch wastewater (adding glucose 20g/L, corn steep liquor 40g/L, ethanol 150mL/L) were investigated through single- factor and orthogonal tests. The suitable culture conditions were as follows:liquid level was 80mL in 250mL triangle bottle, pH4.0, inoculation volume was 9% (V/V) , culture temperature was 28℃ , the the yield of bacterial cellulose reached the peak (4.41g/L) at this time. The bacterial cellulose was verified by FTIR, SEM was used to observe the surface pattern of bacterial cellulose membrane.
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[1] 马道文, 杨劲峰.玉米淀粉生产废水资源化及处理技术研究[J].河北化工, 2010, 33 (7) :74-77. [2] 王煊良.淀粉行业污水处理系统设计及控制策略的研究[D].沈阳:东北大学, 2008. [3] 陆胜民, 贾静静, 杨颖.细菌纤维素发酵工艺与应用研究进展[J].食品与发酵科技, 2010, 47 (1) :27-31. [4] Kenji T, Masashi F, Mitsuo T, et al.Synthesis of Acetobacter Xylinum bacterial cellulose composite and its meth.strength and biodegradability[J].Mokuzai Gakkaishi, 1995, 41 (8) :749-757.
[5] Krystynowicz A, Czaja W, Wiktorowska Jezierska A, et al.Factors affecting the yield and properties of bacterial celluose[J].Journal of Industrial microbiology, 2002, 29:189-195.
[6] Gullo M, Giudici P.Acetic acid bacteria in traditional balsamic vinegar:Phenotypic relevant for starter cultures selection[J].Int J Food Microbiol, 2008:125 (1) :46-53.
[7] Membrane&Separation Technology News Group.Bacterial cellulose metallized for PEMs[J].Membrane&Separation Technology News, 2002, 20 (6) :6.
[8] Mahadevaswamy U R, Anu A.Optimaization of culture for bacterial cellulose production from Gluconacetobacter hansenii UAC09[J].Annals of Microbiology, 2011, 61 (4) :781-787.
[9] Vu Tuan N, Bernadine F, Michael J, et al.Characterization of Cellulose Production by a Cluconacetobacter xylinus Strain from Kombucha[J].Current Microbiology, 2008:57 (5) :449-453.
[10] 谢健健, 洪枫.细菌纤维素发酵原料的研究进展[J].纤维素科学与技术, 2011, 19 (3) :68-77. [11] 宁永成.有机化合物结构鉴定与有机波谱学[M].第二版.北京:科学出版社, 2001, 485-498. [12] Hui-Huang Chen, Li-Chen Chen, Huang-Chan Huang, et al.In situ modification of bacterial cellulose nanostructure by adding CMC during the growth of Gluconacetobacter xylinus[J].Cellulose.2011, 18 (6) :1573-1583.
[13] 谭玉静, 洪枫.细菌纤维素的静态发酵及物理性质研究[J].纤维素科学与技术, 2007, 15 (4) :1-7. [14] 王敏, 朱平, 赵晓霞, 等.细菌纤维素膜的制备与性能[J].功能高分子学报, 2008, 21 (4) :405-410. [15] 王银存, 李利军, 马英辉, 等.细菌纤维素生产及应用研究进展[J].中国酿造, 2011 (4) :20-23. [16] 武志芳, 张霞, 易彬, 等.新型生物材料细菌纤维素的研究进展[J].食品与发酵科技, 2010, 46 (1) :27-30.
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