蛋氨酸及其羟基类似物对肉鸡肌肉蛋白质合成及质构特性的影响
详细信息Effects of methionine and methionine hydroxy analogue on muscle protein synthesis and flesh texture in broiler chickens
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摘要: 目的:比较研究了DL-蛋氨酸（DLM）和蛋氨酸羟基类似物（HMTBA）对肉鸡肌肉蛋白质合成及质构特性的影响。方法:105只1日龄雄性罗斯肉鸡（约49g）随机分为对照组（饲喂基础日粮）和实验组（0.1%DLM组、0.3%DLM组、0.1%HMTBA组以及0.3%HMTBA组,DLM和HMTBA前期添加剂量为0.1%和0.3%,后期添加剂量为0.08%和0.24%）,共7个处理组,每组3个重复,每个重复5只鸡。结果:饲喂0.3%HMTBA可显著提高肉鸡日增重、腿肌重及腿肌率（p<0.05）;与对照组相比,添加0.1%DLM、0.1%HMTBA以及0.3%HTBA可显著提高肌肉RNA含量、蛋白质含量以及RNA/Protein比值（CS）（p<0.05）,且HMTBA效果优于DLM;添加0.3%HMTBA可显著提高肌肉葡萄糖含量及ATPase水平（p<0.05）,显著降低肌肉糖原含量以及肌肉失水率、硬度、咀嚼度和粘结性（p<0.05）。结论:与DLM相比,HMTBA可更好地促进肉鸡肌肉生长,提高肌肉蛋白质合成,具有改善肌肉质构特性,提高肌肉品质的功能。Abstract: The objective of this experiment was to determine the effects of methionine (DLM) , 2 - hydroxy - 4 - (methylthio) butanoic acid (HMTBA) on muscle protein synthesis and flesh texture in broiler chickens. 105 male Ross broiler chickens (49g) were randomly divided into 7 groups (15 birds per group) , with 3 replicate pens per treatment and 5 birds per pen. All broilers were offered the same basal diet with added DLM and HMTBA at levels of 0 (control group) , 0. 1% and 0. 3% (starter phase) , 0. 08% and 0. 24% (finishing phases) . Results:The body weight gain, leg weight and leg muscle rate were significantly higher in 0. 3% HMTBA group (p<0. 05) , as compared to the control group. Additionally, a significant increase of RNA content, protein content and CS ratio were found in 0. 1% DLM, 0. 1% HMTBA and 0. 3% HMTBA groups (p<0. 05) . Moreover, broiler chickens fed with 0. 3% HMTBA had a significant lower glucose and higher glycogen content and ATPase activity in muscle as compared to the DLM group and control group. A significant decreased hardness, gumminess, and chewiness of flesh were also indicated for 0. 3% HMTBA group. Conclusion:Compared with DLM, HMTBA could better enhance protein synthesis, accelerate muscle growth, and improve flesh quality in broiler chickens.
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