五种抗氧化剂对丙烯酰胺引起小鼠氧化损伤和肾损伤的保护作用
详细信息Protective effect of five antioxidants against acrylamide-induced oxidative injury and kidney injury
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摘要: 丙烯酰胺(AA)是一种对人体具有潜在致癌性的物质,可导致细胞遗传物质DNA的损伤。主要研究了五种抗氧化剂(葛根素、木犀草素、染料木素、槲皮素、柚皮素)对AA引起小鼠氧化损伤和肾损伤的保护作用。56只雄性BALB/c小鼠随机分为7组:对照组,AA染毒组,五个抗氧化剂保护组,通过测定血清中还原性谷胱甘肽(GSH)、尿素氮(BUK)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,研究五种抗氧化剂的保护作用。结果表明,与AA染毒组小鼠相比,抗氧化剂保护组小鼠能明显升高小鼠血清中GSH、SOD的含量,降低BUK和MDA的含量。五种抗氧化剂都能不同程度地降低AA引起小鼠氧化损伤和肾损伤,其中染料木素的保护作用最为明显。Abstract: Acrylamide (AA) is a toxic compound that could lead the damage of DNA in cells. In present paper, it was mainly discussed the protective effect of five antioxidants (puerarin, luteolin, genistein, quercetin, and naringenin) on AA-induced oxidative injury and kidney damage. Fifty-six male Balb/c mice were randomly divided into seven groups, Control group, AA group, five antioxidant groups. The oxidative injury and kidney injury were evaluated by the indexes of serum , including glutathione (GSH) , superoxide dismutase (SOD) , malondialdehyde (MDA) and serurn urea nitrogen (BUN) . The results showed that compared with AA group, antioxidants groups could increase the content of GSH and SOD activity and decrease the levels of MDA and BUN in the serum of the mice significantly. Genistein had significant protective effects on AA-induced oxidative injury and kidney injury was most obviously.
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