尿素包合法富集纯化杜仲籽油α-亚麻酸的工艺优化
详细信息Optimization of enrichment and purification technology of α-linolenic acid in Eucommia seed oil by the urea adduction fractionation method
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摘要: 采用二次正交旋转组合设计,对尿素包合法富集纯化杜仲籽油中α-亚麻酸的工艺进行了优化研究。在单因素实验的基础上,以α-亚麻酸纯度为考察指标,考察了尿素与脂肪酸质量比、95%乙醇与脂肪酸质量比、包合温度、包合时间等四个实验因素对α-亚麻酸富集效果的影响,建立了二次多元回归方程预测模型。实验结果表明α-亚麻酸最佳富集工艺条件为:尿素与脂肪酸质量比为3:1,95%乙醇与脂肪酸质量比为9:1,包合温度为-9.0℃,包合时间为17.0h。在此最佳富集条件下,α-亚麻酸纯度可提高至82.63%。尿素包合法是富集纯化杜仲籽油α-亚麻酸的有效方法。Abstract: Quadratic rotation-orthogonal combination design was applied to optimize the enrichment and purification techniques of α-linolenic acid in Eucommia ulmoides seed oil by the method of urea adduction fractionation. By taking α-linolenic acid purity as the investigation index, the effects of four process parameters named ratio of urea to fatty acid, ratio of 95% ethanol to fatty acid, inclusion temperature and inclusion time on the enrichment results were investigated and the two times multiple regression equation model of α-linolenic acid purity was established based on the single factor experiments. The results showed that the optimum levels of the four parameters were as follows:3:1, 9:1, -9. 0℃ and 17. 0h, respectively. Under the optimum enrichment conditions, the purity of α-linolenic acid was up to 82. 63%. Urea adduction fractionation was an effective method for the enrichment of α-linolenic acid in Eucommia ulmoides seed oil.
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