Extraction and anti-allergy activity in vitro of cAMP from Jujube
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摘要: 大枣中环磷酸腺苷（cAMP）的含量极其丰富,具有调节免疫、抑制哮喘等生理作用。本研究采用闪式提取法提取早脆王枣中的cAMP,利用大孔树脂进行纯化,并对其体外抗过敏活性进行研究。正交实验结果表明,最优提取条件为:60%乙醇、料液比1:20、提取次数3次、提取时间40s,此条件下cAMP的提取率达（149.5±3.6）μg/g。纯化条件为提取液浓度20μg/mL,以1.5BV/h流速上样,上样体积70mL,以40%乙醇洗脱,流速为1BV/h。在此条件下,所获大枣cAMP提取物的纯度可达68.5%±3.4%。利用Elson-Morgan法对所获大枣cAMP提取物进行抗过敏活性评价,其透明质酸酶抑制率可达96.2%±4.1%,表明具有潜在的功能应用前景。
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关键词:
- 大枣; /
- 环磷酸腺苷; /
- 闪式提取; /
- 抗过敏活性; /
- Elson-Morgan法;
Abstract: The content of cyclic adenosine monophosphate (cAMP) in jujube is extremely abundant, and it has many physiological functions such as immune regulation and asthma inhibition. In the present study, cAMP was extracted from Zizyphus Jujuba Mill Zaocuiwang by using flash extraction method, and then purified by macroporous adsorption resin. The anti-allergic activity in vitro of the purified extract was further investigated. The result of orthogonal experiment indicated the following optimal parameters of the extraction process:the concentration of ethanol was 60%, the ratio of sample to extracting solution was 1:20, extraction times were 3 times, and the extraction duration was 40s. The extraction ratio of cAMP was (149. 5±3. 6) μg/g under the optimal extraction process. The cAMP extract with a purity of 68. 5%±3. 4% was obtained by purification in the following condition:the concentration of the extract was 20μg/mL, the flow rate was 1. 5BV/h, the sample volume was 70mL, and the elution rate was 1BV/h using 40% ethanol. The anti-allergic activity of the cAMP extract evaluated by the Elson-Morgan method reached up to 96. 2%±4. 1% (expressed by inhibition on the activity of hyaluronidase) , suggesting a potential in its functional application. -
[1] 高续春.枣渣中有效成分的提取分离研究[D].西安:西北大学, 2007. [2] Nikolaus A.The current status of cyclic AMP in high plants[J].Annual Review of Plant Physiology, 1977, 2 (8) :123-132.
[3] Schwede F, Maronde E, Genieser H, et al.Cyclic nucleotide analogs as biochemical tools and prospective drugs[J].Pharmacol&Therapeutics, 2000, 87 (2-3) :199-226.
[4] Marin D, Dunphy G B, Mandato C A.Cyclic AMP affects the haemocyte responses of larval Galleria mellonella to selected antigens[J].Journal of Insect Pysiology, 2005, 51 (5) :575-586.
[5] Yuen L P, Wong A S T, Auersperg N.Gonadotropins Regulate N-cadherin-mediated Human Ovarian Surface Epithelial Cell Survival at Both Post-translational and Transcriptional Levels through a Cyclic AMP/Protein Kinase A Pathway[J].Journal of Biological Chemistry, 2005, 280 (15) :15438-15448.
[6] Fabiana S, Giorgio F, Cecilia F, et al.Bordetella pertussis Inhibition of Interleukin-12 (IL-12) p70in Human Monocyte-Derived Dendritic Cells Blocks IL-12p35through Adenylate Cyclase Toxin-Dependent Cyclic AMP Induction[J].Infection and Immunity, 2006, 74 (5) :2831-2838.
[7] Fedyk E R, Adawi A, Looney R J, et al.Regulation of IgE and Cytokine Production by cAMP:Implication for Extrinsic Asthma[J].Clinical Immunology and Immunopathology, 1996, 81 (2) :101-113.
[8] 崔志强, 孟宪军.微波辅助萃取冬枣环磷酸腺苷工艺研究[J].食品科学, 2007, 28 (4) :163-166. [9] Wang H H, Lin Y L, Liao J F, et al.The anti-amnesic effects of the water extract of Jujubae Fructus[J].The Chinese Pharmaceutical Journal (Taipei) , 2003, 55 (5) :361-369.
[10] 林琎, 杜磊, 罗万春.几种常用分离提取设备的分离提取技术[J].实验科学与技术, 2010, 8 (3) :4-6. [11] 尤妍.哈密大枣环磷酸腺昔提取纯化工艺研究[D].乌鲁木齐:新疆农业大学, 2011. [12] 王立霞, 陈锦屏, 张娜, 等.和田玉枣中cAMP提取工艺的研究[J].食品工业科技, 2009, 30 (10) :234-236. [13] 尤逢惠.大枣cAMP的分离纯化工艺研究[D].合肥:合肥工业大学, 2007. [14] 王荔, 亓树艳, 莫晓燕.大枣环磷酸腺苷提取纯初步研究[J].食品科技, 2012, 37 (4) :191-194. [15] 赵丰丽, 李洁荣, 健秀.黄皮叶不同溶剂提取物抗过敏活性研究[J].食品工业科技, 2009, 30 (1) :110-112, 115.
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