Study on synthesis and antibacterial activity of dihydromyricetin-Ni complexes
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摘要: 以黄酮类二氢杨梅素和乙酸镍盐为原料,合成二氢杨梅素-镍配合物,通过红外光谱、紫外光谱、差热-热重分析对配合物进行表征。采用试管二倍稀释法研究配体二氢杨梅素及其镍配合物的抗菌活性。结果表明合成的产物确证为二氢杨梅素-镍配合物,配合物表现出较强的抑菌和杀菌作用,对白假丝酵母菌MIC为6.25～12.5μg/mL、MBC为6.25～25μg/mL。其抗酵母菌能力明显强于配体二氢杨梅素,但对细菌抑制效果不明显。Abstract: Dihydromyricetin-Ni (Ⅱ) was prepared with using dihydromyricetin and Ni (CH3COO) 2 .The compex was characterized by UV spectra, IR spectra and TGA spectra.The antibacterial activity of the dihydromyricetin and the complex were compared by tube double dilution method.The results showed that the synthetic product was confirmed as dihydromyricetin-nickel complexes.The complexes showed strong bacteriostatic and bactericidal effect.The minimum antimicrobial dosage of dihydromyricetin-Ni (Ⅱ) against Candida albicans was 6.25～12.5μg/mL and the germicidal dosage was 6.25～25μg/mL.The results showed dihydromyricetin-Ni (Ⅱ) against Candida albicans was stronger than that of dihydromyricetin, but not obvious inhibitory effect on bacteria.
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[1] 曹治权.中药药效的物质基础和作用机理研究新思路 (1) —中药中化学物种形态和生物活性关系的研究[J].上海中医药大学学报, 2000, 14 (1) :36-40. [2] 刘文胜, 罗维早, 张志荣, 等.中药研究的新学说——中药配位化学[J].华西药学杂志, 2001, 16 (4) :293-294. [3] Qiukai Zheng, Linli Xu, Linhua Zhu, et al.Preliminary investigations of antioxidation of Dihydromyricetin in polymers[J].Bull Mater Sci, 2010, 33 (3) :273-275.
[4] Ying Xu, Fei Wang, Le Wang, et al.Baocheng Yang, Baoxian Ye, Sensitive voltammetric sensor of dihydromyricetin based on Nafion/SWNT-modified glassy carbon electrode[J].J Solid State Electrochem, 2012, 16:1473-1480.
[5] Marchela Pandelova, Walkiria Levy Lopez, Bernhard Michalke, et al.Ca, Cd, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn contents in baby foods from the EU market:Comparison of assessed infant intakes with the present safety limits for minerals and trace elements[J].Journal of Food Composition and Analysis, 2012, 27 (2) :120-127.
[6] Yan Zhang, Gladyshev, Vadim N.General Trends in Trace Element Utilization Revealed by Comparative Genomic Analyses of Co, Cu, Mo, Ni, and Se[J].Journal of Biological Chemistry, 2010, 285 (5) :3393-3405.
[7] 李海霞, 陈丽珍, 翟锐锐, 等.薄层色谱法在二氢杨梅素螯合锰合成工艺中的应用[J].海南医学院学报, 2011, 17 (4) :445-447, 455. [8] Chohan Z H, Arif M, Sarfraz M.Metal-based antibacterial and antifungal amino acid derived Schiff based:Their synthesis, characterization and in vitro biological activeity[J].Appl Organomet Chem, 2007, 21 (4) :294-302.
[9] 周美锋, 何其庄, 费菲.纳米稀土谷氨酸咪唑三元配合物的合成、表征及抗菌活性研究[J].中国稀土学报, 2007, 25 (5) :549-555. [10] 冯小强, 李小芳, 杨声, 等.壳聚糖镧配合物的制备、表征及其抑菌性能[J].食品工业科技, 2010, 31 (2) :304-310. [11] 萧力争, 银霞, 刘素纯, 等.二氢杨梅素抗菌活性研究[J].食品科技, 2008 (4) :140-143. [12] 杨书珍, 张友胜, 宁正祥, 等.二氢杨梅素对几种食品常见菌的抑制效果[J].天然产物研究与开发, 2003, 15 (1) :40-42.
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