人参皂苷生物转化及固体发酵工艺研究进展
详细信息Research progress in biotransformation of ginsenosides and solid state fermentation by microorganism
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摘要: 研究表明,人参皂苷在体外可以转化成多种稀有皂苷,它们是在生物体内发挥药理活性的关键物质。微生物转化法及固体发酵技术是工业化生产稀有人参皂苷的基础。文中针对皂苷生物转化研究概况进行综述,探讨了固体发酵工艺条件,并对目前人参皂苷生物转化研究中存在的问题及今后的主要发展方向进行了展望。Abstract: According to the previous studies, ginseng saponin can be converted into a variety of rare ginsenoside in vitro.They played the key role of pharmacological activity in vivo substances.The microbial transformation and solid -state fermentation technology is the basis for industrial production of rare ginsenoside.In this paper, the studies on ginsenoside biotransformation research survey and ginseng solid fermentation process were explored, and the existing problems and the main development direction of ginseng biotransformation were forecasted
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[1] 王毅, 刘铁汉, 王巍, 等.肠内菌群对人参皂苷Rg1的代谢转化作用的研究[J].中国中药杂志, 2001, 36 (3) :188-190. [2] 窦德强, 靳玲, 陈英杰.人参的化学成分及药理活性的研究进展与展望[J].沈阳药科大学学报, 1999, 16 (2) :151-156. [3] Wang YZ, Chen J, Chu SF, et al.Improve-ment of memory in mice and increase of hippocampal excitability in rats by ginsenoside Rg1’s metabolites ginsenoside Rh1and protopanaxatriol[J].Pharmacol Sci, 2009, 109:504-510.
[4] 弓晓杰.人参皂苷酶代谢产物化学修饰及其抗癌活性研究[D].长春:吉林农业大学, 2004. [5] Hasegawa H, Sung J H, Matsumiya S, et al.Reversal of daunomycin and vinblastine resistance in multidrug-resistant P388leukemia in vitro through enhanced cytotoxicity by triterpenoids[J].Planta Med, 1995, 60 (5) :409-413.
[6] Hasegawa H.Proof of the mysterious efficacy of ginseng:basic and clinical trials:metabolic activation of ginsenoside:deglycosylation by intestinal bacteria and esterification with fatty acid[J].J Pharmacol Sci, 2004, 95 (2) :153-157.
[7] Hasegawa H, Sung J H, Matsumiya S, et al.Main ginseng saponin metabolites formed by intestinal bacteria[J].Planta Med, 1996, 62:453-457.
[8] 徐绥绪, 谭俊婧.红参中特征性成分人参皂Rh2的分离与鉴定[J].中草药, 1987, 18 (9) :36-38. [9] 吕永俊, 徐绥绪.人参皂苷的化学研究方法[J].沈阳药学院学报, 1985, 2 (1) :63-80. [10] 马建标, 赵存祥, 范云鸽.绞股蓝皂苷酸水解次生苷元的分离与鉴定[J].化学学报, 1993, 51 (4) :708-712. [11] 陈业高, 黄荣, 桂世鸿, 等.三七叶甙制备抗癌活性成分20 (R) -人参皂甙Rh2和人参皂甙Rg3[J].化学研究与应用, 2004, 16 (1) :69-70. [12] Han B H, Park M H, Han Y N, et al.Degradation of Ginseng Soponins Under Mild Acidic Conditions[J].Planta Med, 1982, 44:146-149.
[13] 刘志辉, 李琳, 钱芳.醋酸水解三七总皂苷制备人参皂苷Rg3工艺的优选[J].中国医院药学杂志, 2009, 29 (11) :881-884. [14] Bae Ea, HanM j, Kim E j, et al.Transformation of ginseng saponins to ginsenoside Rh2by acids and human intestinal bacteria and biological activities of the transformants.[J].Arch Pharm Res, 2004, 27 (1) :61-67.
[15] 马晓宁, 柴瑞华, 赵余庆.西洋参茎叶皂苷水解产物中稀有抗肿瘤成分的化学研究[J].中草药, 2008, 39 (9) :1291-1294. [16] Chi H, Kim DH, Ji GE.Transformation of Ginsenosides Rb2and Rc from Panax ginseng by food microorganisms[J].Biol Pharm Bull, 2005, 28 (11) :2102-2105.
[17] 宋长春, 徐景达, 陈燕萍, 等.20 (S) -G-Rh2的制备方法, 其药物组合物及应用[P].中国专利:1225366, 1999, 8, 11. [18] 陈燕萍, 孟勤, 宋长春, 等.20 (S) -原人参二醇组皂甙的制备及其转化制取人参皂苷Rh2[J].中国药学杂志, 1997, 32 (5) :273. [19] 陈业高, 吕瑜平, 桂世红, 等.三七叶甙制备原人参二醇及其差向异构体[J].精细化工, 2003, 20 (7) :425-426. [20] Cha B C, Lee S G.Preparation of ginsenoside-Rh2from dammarane saponins of Panax ginseng leaves[J].Yakhak Hoechi, 1994, 38 (4) :425-429.
[21] 金凤燮, 鱼红闪, 赵立亚.酶法生产稀有人参皂甙及其产物成分的分析[J].大连轻工学院学报, 2002, 21 (3) :112-115. [22] KIM Bohye, LEE Sunyi, CHO Hyojin, et al.Biotransformation of Korean Panax ginseng by Pectinex[J].Biol Pharm Bull, 2006, 29 (12) :2472-2478
[23] 吕永俊, 徐绥绪.人参皂苷的化学研究方法[J].沈阳药学院学报, 1985, 2 (1) :63-82. [24] Jiang BH, Han Y, Zhao YQ.Optimization of enzymatic translation for preparation ginsenoside compound K in total saponins of Panax notoginseng[J].Chin Tradit Herb Drugs, 2004, 35:986-988.
[25] 李学龙, 富瑶瑶, 孙斯宜.Arthrobacter sp.3胞外酶转化人参二醇类皂苷生成Rg3的反应条件[J].大连工业大学学报, 2010, 29 (6) :396-398. [26] 侯金刚, 李伟, 郑毅男.酶解法转化人参皂苷Rh1及其含量测定[J].中国中药杂志, 2009, 34 (23) :3030-3033. [27] 童庆宣, 陈良华, 明艳林.稀有人参皂苷IH901酶法转化与制备研究[J].天然产物研究与开发.2009 (21) :1039-1044. [28] 韩颖、胡筱敏、赵余庆, 甘蔗镰孢霉与工业用酶制剂对三七茎叶有效成分转化作用的比较[J].生态学杂志, 2008, 27 (8) :1434-1437. [29] 宋建国, 肖永坤, 王东明.人参皂苷Re酶转化的F1皂苷等产品的分离提纯[J].大连工业大学学报, 2012, 31 (3) :157-160. [30] WANG Dongming, YU Hongshan, SONG Jianguo, et al.A novel ginsenosidase from an Aspergillus strain hydrolyzing6-O-glycosides of protopanaxatriol-type ginsenosides, named ginsenosidase typeⅣ[J].Journal of Microbiology andBiotechnology, 2011, 21 (10) :1057-1063.
[31] Eun-Ah Bae.Metabolism of20 (S) -and20 (R) -Ginsenoside Rg3by Human Intestinal Bacteria and Its Relation to in Vitro Biological Activities[J].Biol PharmBull, 2002, 25 (1) :58-63.
[32] Hasegawa H, Sung JH, Benno Y, Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins[J].Planta Med, 1997Oct;63 (5) :436-40.
[33] Han Y, Sun B S, Hu XM, et al.Tansformation of bioactive compound by Fusarium sacchari fungus isolated from the soil cultivated ginseng[J]Agric Food Chem, 2007 (55) :9373-9379.
[34] 包海鹰, 李磊, 昝立峰, 等.黑根霉对人参皂苷Re的生物转化[J].菌物学报, 2010, 29 (4) :548-554. [35] 贾翠英, 张玉辉, 魏均玲.海藻酸钠包埋的酵母菌转化人参皂苷Rb1的研究[J]东北农业大学学报.2010, 41 (9) :60-66. [36] Zhou W, Yan Q, Li J.Y, et al.Biotransformation of Panaxno to ginseng saponins into ginsenoside compound K production by Paecilomyces bainier sp.229[J].J Appl Microbiol, 2008 (104) :699-706.
[37] Li Ye, Chao-Qun Zhou, Wei Zhou, et al.Biotransformation of ginsenoside Rb1to ginsenoside Rd by highly substrate-tolerant Paecilomyces bainier229-7[J].Bioresource Technology, 2010, 101:7872-7876.
[38] 金艳, 金香梅, 尹成日.微生物转化人参皂苷Rb1为稀有皂苷F2[J].延边大学农学学报, 2011, 33 (3) :112-116. [39] 张一.人参果皂苷的微生物转化研究[D].吉林:吉林农业大学生命科学学院, 2008. [40] 王兴红李祺德.微生物发酵中药应成为中药研究的新内容[J].中草药, 2001, 32 (3) :267-268. [41] 刘逆夫, 娄勇军, 王伶俐.中药克癀胶囊药用原料的固态发酵生产工艺研究[J].中南药学, 2007, 5 (4) :314-316. [42] Bong-Gwan Kim, Sin-Yang Choi, Mi-Ryung Kim.Changes of ginsenosides in Korean red ginseng fermented by Lactobacillus plantarum M1[J].Process Biochemistry, 2010, 45:1319-1324.
[43] Kalaiselvi Senthil, V Veena, M.Mahalakshmi.Microbial conversion of major ginsenoside Rb1to minor ginsenoside Rd by Indian fermented food bacteria[J].African Journal of Biotechnology2009, 24 (8) :6961-6966.
[44] 毕华.大型担子菌L10固体发酵人参实验研究[D].吉林:长春中医药大学, 2010. [45] 付建国.人参皂苷微生物转化的研究.[D].吉林:吉林农业大学中药材学院, 2004. [46] 田翠.三七皂苷转化菌株的筛选及其转化产物的分离纯化与鉴定[D].上海:上海师范大学, 2008. [47] 庄毅.真菌药用“固体发酵”上游工程建设基本研究方法[J].中国食用菌, 2009, 28 (4) :8-9. [48] LI Lei, ZAN Li-Feng, WANG Chun-Ling.Ginsenoside Re biotransformed by Rhizopus sp[J].Mycosystema, 2010, 29 (4) :548-554.
[49] 许兰.发酵红参制品的制备方法:中国, 201010151767.6[P].2010-04-21. [50] 李国红, 张克勤, 沈月毛.枯草芽孢杆菌对50种中药的发酵及抗菌活性检测[J].中药材, 2006, 29 (2) :154-157.
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