Study on mechanism and inhibiting method of darkening in whole-wheat fresh noodle
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摘要: 以全麦面粉为原料制作鲜湿面条,追踪其褐变过程,研究其褐变机制,并尝试采用超声波与抗氧化剂联合处理的灭酶方法抑制面条中的褐变反应。结果表明:全麦鲜湿面的24h内的褐变过程可以分为三个阶段;多酚氧化酶酶促反应是影响全麦鲜湿面褐变的主要因素之一;超声波与抗氧化剂联合处理的方法可以有效抑制多酚氧化酶(PPO)的酶活,控制褐变的发生,并且短时间(2min之内)超声处理不会对全麦面粉中矿质元素的含量产生显著影响(p>0.05)。Abstract: Whole-wheat flour was used as material to produce fresh noodles.The experiment was designed to understand the process and the mechanism of darkening and attempt to inhibit browning by using combination of ultrasound and antioxidants.Results showed that there were three stages in the process of darkening in 24 hours.PPO enzymatic reaction was one of major factors influencing darkening in whole-wheat fresh noodles.The combined treatment with ultrasound and antioxidant was an effective method to reduce PPO activity and inhibit darkening process in whole-wheat fresh noodles, ultrasonic treatment in 2min showed no significant effect on the content of minerals in whole-wheat flour in this experiment (p>0.05) .
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Keywords:
- whole-wheat fresh noodle /
- darkening /
- ultrasound /
- antioxidant
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[1] 屈凌波.谷物营养与全谷物食品的研究开发[J].粮食与食品工业, 2011, 18 (5) :7-9. [2] Hou G, Kruk M.Asian noodle technology[J].American Institute of Baking Technical Bullein, 1998 (20) :1-10.
[3] Jiang H, Martin J, Okot-Kotber M, et al.Color of WholeWheat Foods Prepared from a Bright-White Hard Winter Wheat and the Phenolic Acids in Its Coarse Bran[J].Journal of Food Science, 2011, 76 (6) :846-852.
[4] Asenstorfer R E, Appelbee M J, Mares D J.PhysicalChemical Analysis of Non-Polyphenol Oxidase (Non-PPO) Darkening in Yellow Alkaline Noodles[J].Journal of Agricultural and Food Chemistry, 2009, 57 (12) :5556-5562.
[5] 梁华, 钮琰星, 黄凤洪, 等.超声波在食品工业上的应用[J].食品工业科技, 2008, 29 (7) :293-296. [6] Anderson J V, Morris C F.An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity[J].Crop Science, 2001, 41 (6) :1697-1705.
[7] Singleton V L, Orthofer R, Lamuela-Raventos R M.Analysisof total phenols and other oxidation substartes and antioxidants by means of Folin-Ciocalteu reagent[J].Methods Enzymol, 1999, 299:152-178.
[8] Asenstorfer R E, Appelbee M J, Mares D J.Impact of Protein on Darkening in Yellow Alkaline Noodles[J].Journal of Agricultural and Food Chemistry, 2010, 58 (7) :4500-4507.
[9] Mason T J, Lorimer J P, Baters D M, et al.Dosimetry in sonochemistry:the use of aqueous terephthalate ion as a fluorescence monitor[J].Ultrasonics Sonochemistry, 1994 (1) :91-95.
[10] Arias E, Gonzalez J, Peiro J M, et al.Browning prevention by ascorbic acid and 4-hexylresorcinol:Different mechanisms of action on polyphenol oxidase in the presence and in the absence of substrates[J].Journal of Food Science, 2007, 2 (9) :464-470.
[11] Verma B, Hucl P, Chibbar R N.Phenolic acid composition and antioxidant capacity of acid and alkali hydrolysed wheat bran fractions[J].Food Chemistry, 2009, 116 (4) :947-954.
[12] Prat M L, Lopez-Gonzalvez A, Ruiz M A, et al.Ultrasoundassisted extraction for rapid determination of Zn, Cu, Fe, Mg and Mn in liver of diabetic rats under different antioxidant treatments[J].Journal of Pharmaceutical and Biomedical Analysis, 2009, 49 (4) :1040-1044.
[13] Filgueiras A V, Capelo J L, Lavilla I, et al.Comparison of ultrasound-assisted extraction and microwave-assisted digestion for determination of magnesium, manganese and zinc in plant samples by flame atomic absorption spectrometry[J].Talanta, 2000, 53:433-441.
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