Study on microwave-assisted extraction and purification of flavonoids by macroporous resins from Corylus heterophylla leaves
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摘要: 通过单因素实验对影响微波辅助提取榛子叶中黄酮类化合物的主要因素进行了考察,确定了最佳提取条件是:乙醇浓度为55%（V/V）,料液比为1∶35（g∶mL）,微波温度80℃,微波功率300W,提取时间5min。并对提取出的黄酮物质进行纯化,通过比较AB-8、D101等八种大孔吸附树脂的静态吸附和解吸性能,筛选出适合纯化榛子叶黄酮的树脂类型;采用动态法对样品液流速、样品液pH、解析液pH和解析液乙醇浓度进行了研究;同时采用高效液相色谱法对纯化前后的黄酮进行了分析比较。结果表明,D101型大孔树脂对榛子叶黄酮具有较佳的吸附和解吸性能,最佳纯化条件为:样品液流速为2BV/h、样品液pH4、解析液pH4,解析液乙醇浓度为70%。在此条件下,纯化后的榛子叶黄酮含量提升至54.7%。Abstract: Microwave-assisted extraction (MAE) of flavonoids from Corylus heterophylla leaves was investigated.Effects of several experimental parameters, such as solvent concentration, temperature, microwave power, and microwave irradiation time, on the extraction yield of flavonoids from Corylus heterophylla leaves were evaluated.The optimal conditions of MAE used were extraction with 55% ethanol as solvent, solid-liquid ratio 1∶35 (g∶ mL) , a microwave power of 300W, 80℃, and microwave irradiation time 5min.Thereafter, the macroporous adsorbent resin was used to enrich the flavonoids in the crude extract.The adsorption and desorption of eight macroporous adsorbent resins for flavonoids were compared.The optimal adsorbent was D101.The optimal adsorption /desorption conditions were determined as follows: pH4 for sample solution, the extract solution passed through the column at a flow rate of 2BV/h, the column was washed with 3BV, pH4, 70% alcohol at a flow rate of 2BV/h.The purity of flavonoids was increased to 54.7%.
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Keywords:
- Corylus heterophylla leaves /
- flavonoids /
- extraction /
- macroporous resin /
- purification
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