Photosynthesis oxidases combined chemical oxidation used for delignification in lignocellulose
-
摘要: 光合氧化酶用于木质素降解,因受固态木质素与酶接触限制降解效率较低。TiO2、H2O2/Fe2+处理构树木质纤维后,再以光合氧化酶或漆酶进行酶促降解,明显提高了木质素的降解效率。结果显示,以纳米TiO2或H2O2/Fe2+联合处理构树木质纤维1.5h后再以光合氧化酶进行木质素降解1.5h,木质素的降解效率较单独以光合氧化酶降解3h分别提高36.0%和28.8%,以纳米TiO2或H2O2/Fe2+联合处理构树木质纤维1.5h后,再以漆酶降解1.5h,木质素的降解效率较单独以漆酶降解3h分别提高92.4%和85.0%。纳米TiO2和H2O2/Fe2+与光合氧化酶或漆酶联合作用,可有效提高木质素的酶促降解效率。Abstract: The degradation of lignin by enzyme had a low degradation efficiency because it suffered restriction by the contact between solid state lignin and enzyme, which could be improved by combining the lightly toxic and tractable oxidant such as TiO 2 and H 2 O 2 /Fe2 +and oxidase into the process of degradation.Brousso xylem pretreatmented by TiO 2 or H 2 O 2 /Fe2 +then were degraded by photosynthesis oxidases or laccase in this paper.The results showed that papyrifera xylem fibre pretreatmented by nanometer- sized TiO 2 or H 2 O 2 /Fe2 +for 1.5h and then by photosynthesis oxidases for 1.5h, the degradation efficiency of lignin increased 36.0% or 28.8% respectively compared to those were degraded by photosynthesis oxidases for 3h alone.Conducted by nanometer- sized TiO 2 for 1.5h and then degraded by laccase for 1.5h, the degradation efficiency of lignin increased 92.4% or 85.0% compared to those were degraded by laccase for 3h.Consequently the degradation efficiency of lignin by photosynthesis oxidases or laccase could be facilitated efficiently by joint action with nanometer- sized TiO 2 or H 2 O 2 /Fe2 +.
-
Keywords:
- photosynthesis oxidases /
- laccase /
- nanometer TiO2 /
- H2O2 /Fe2+ /
- degradation of lignin
-
[1] Higuchi T.Microbial degradation of lignin:Role of lignin peroxidase, manganese peroxidase, and laccase.Proceedings of the Japan Academy, Series B[J].Physical and Biological Sciences, 2004, 80 (5) :204-215.
[2] Mayer A M, Staples R C.Laccase:new functions for an old enzyme[J].Phytochemistry, 2002, 60 (6) :551-565.
[3] 池玉杰, 伊洪伟.木材白腐菌分解木质素的酶系统-锰过氧化物酶、漆酶和木质素过氧化物酶催化分解木质素的机制[J].菌物学报, 2007, 26 (1) :153-160. [4] 万云洋, 杜予民.漆酶结构与催化机理[J].化学通报, 2007, 9:662-670. [5] 韩广业, 李淑芹, 唐崇钦, 等.光合放氧复合物结构及其放氧机理的研究[J].化学进展, 2004, 16 (2) :184-195. [6] 熊燕飞, 甘玮, 王洋, 等, 光反应酶系统脱木质素作用研究[J].食品工业科技, 2013, 34 (7) :182-185. [7] 甘玮.光-酶联合用于木纤维脱木质素新方法及工艺研究[D].武汉:武汉理工大学, 2012. [8] 李晓洁.完整叶绿体提取方法的优化讨论[M].济南:科技信息, 2010:761-762. [9] K Mogyorósi, I.Dékány.Preparation and Characterization of Clay Mineral Intercalated Titanium Dioxide Nanoparticles[J].Langmuir, 2003, 19 (7) :2938-2946.
[10] Maira A J, Yeung K L, Lee C Y, et al.Size effects in gasphase photo-oxidation of trichloroethylene using nanometer-sized TiO2 catalysts[J].J Catal, 2000, 192:185-196.
[11] 周武艺, 唐绍裘, 万隆, 等.纳米TiO2光催化降解有机物的机理及其影响因素的研究[J].中国陶瓷工业, 2OO3, 10 (5) , 26-29. [12] Zeng G M, Yu H Y, Huang H L, et al.Laccase activities of soil inhabiting fungus Penicillium simplicissimum in relation to lignin degradation[J].World Journal of Microbiology and Biotechnology, 2006, 22:317-324.
计量
- 文章访问数: 118
- HTML全文浏览量: 17
- PDF下载量: 106
下载:
