Study on extraction and separation of octopamine from fish sauce
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摘要: 本文采用静态吸附和动态吸附相结合的方法,综合考虑吸附量、解吸率等因素,研究硅胶分离提取鱼露中章鱼胺的吸附性能和洗脱参数。结果表明:60100目硅胶对鱼露中章鱼胺有较好的吸附分离效果,静态饱和吸附量达3.837mg·g-1（干硅胶）,30%的乙醇水溶液为最佳洗脱剂,洗脱率达90.48%;60100目硅胶对鱼露中章鱼胺动态吸附的最佳上样量为4BV,用1.1BV洗脱液可基本洗脱完全。得到的洗出液采用HPLC分离系统进行精制,真空冷冻干燥后得到白色粉末状固体。将此白色固体与标准品章鱼胺在同样的条件下进行质谱分析,可证明得到的白色粉末状固体为纯度较高的天然章鱼胺。Abstract: In this study, the adsorption and elution parameters was studied during extraction and separation of octopamine from fish sauce, while the static and dynamic adsorption was combined and adsorption and desorption rate were also considered.The results demonstrated that 60100 mesh silica gel had high efficiency in separate adsorption with the static saturated adsorption of 3.837mg·g- 1 (dry silica gel) .30% ethanol was as eluent with its elutive power 90.48%.The optimal value of the dynamic adsorption of octopamine in fish sauce on 60100 mesh silica gel was 4BV, and when the volume of eluent was 1.1BV, the eluting process was basically completed.The eluate was refined by a HPLC separation system.White solid powder was obtained after vacuum freeze drying.This white powder and octopamine standard was analyzed by mass spectrometer under the same condition.It can be proved that the white powder was natural octopamine with high purity.
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Keywords:
- octopamine /
- fish sauce /
- separation /
- extraction /
- silica gel
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