Optimization the L-carnitine determination by spectrophotometric method and comparison of content in the yak organs
-
摘要: 对牦牛脏器中左旋肉碱的分光光度法测定进行优化,并对其脏器中左旋肉碱含量进行分析。在单因素实验的基础上,以加酶反应时间、缓冲体系pH、酶反应温度为自变量,以吸光度为响应值,进行响应面分析。确定最优工艺条件为:酶反应温度37℃、缓冲体系pH7.6、加酶反应时间16min,在此条件下,左旋肉碱吸光度值为0.321。通过对牦牛心、肝、肺、肾中左旋肉碱测定值的分析发现,肝脏中左旋肉碱含量最高,为28.35mg/kg;肺脏中左旋肉碱含量为22.75mg/kg;心脏中含左旋肉碱14.61mg/kg;肾脏中左旋肉碱的含量最低,为5.22mg/kg。Abstract: The spectrophotometric determination method about L-carnitine in organs of yak was optimized and the content of L-carnitine in the yak organs was analyzed in this paper. Based on the single factor experiment, the response surface methodology was used to select the enzyme reaction time, pH of buffer system and enzyme reaction temperature. These three was shown as variables, and absorbance as response value. The optimum conditions were as follows:the enzyme reaction temperature was 37℃, pH was 7.6 and enzyme reaction time was 16 minutes. Under this condition, the absorbance of L-carnitine was 0.321. The measured value of yak heart, liver, lung and kidney was analyzed under the optimum condition. And the result was shown that the highest L-carnitine yield of 28.35mg/kg was achieved from liver, then was lung of 22.75mg/kg and heart of 14.61mg/kg, kidney had the lowest content of 5.22mg/kg.
-
Keywords:
- spectrophotometric method /
- yak viscera /
- L-carnitine /
- method optimization /
- content analysis
-
[1] 张凤清, 张丽君, 周长民.左旋肉碱的制备方法[J].当代化工, 2008, 23 (10) :100-120. [2] 孙娜, 余群力, 曹晖.超声波辅助高氯酸法提取牛肝中左旋肉碱工艺优化[J].农业工程学报, 2012, 28 (21) :280-285. [3] 曾云, 丁峰.左旋肉碱合成与前景浅析[J].广东化工, 2012, 39 (1) :71-72. [4] 陈骋, 张丽, 韩玲, 等.牦牛不同脏器中牛磺酸提取工艺优化及含量分析[J].食品工业科技, 2013, 34 (2) :83-86. [5] 毕伟健, 黄金发.左旋肉碱在食品中的应用[J].广西轻工业, 2010, 10 (8) :7-20. [6] Knuttel G S, Harmeyer J.The determination of L-carnitine in several food samples[J].Food Chemistry, 2007, 105:793-804.
[7] 杨艳, 吴广枫.高效液相色谱-串联质谱法测定葡萄果实中的乙酰辅酶A[J].色谱, 2013, 31 (1) :33-37. [8] 魏任雄, 熊彬.循环酶法测定血浆同型半胱氨酸的方法学评价[J].中国卫生检验杂志, 2010, 20 (6) :1474-1475. [9] 王天西, 熊文明, 林梦勇.分光光度法测定乳粉中左旋肉碱含量[J].福建分析测试, 2012, 21 (1) :52-56. [10] 顾国龙.酶法测定的影响因素及对策[J].医学综述, 2006, 12 (19) :1209-1211. [11] 孟宪华, 邢冬红, 郑治纲.酶循环法测定血清肉碱浓度及临床意义[J].陕西医学杂志, 2001, 30 (10) :585-587. [12] Takahashi M, Shigeru U, Hideo M, et al.Carnitine determination by an enzymatic cycling method with carnitine dehydrogenase[J].Journal of Food Science, 1994, 40 (5) :817-821.
[13] 王长瑞, 于长青, 王立东, 等.超声波酶法制备血红素工艺条件的研究[J].黑龙江八一农垦大学学报, 2008, 20 (4) :67-71. [14] 邹雨佳, 聂实践, 李东.L-肉碱的检测方法[J].生物工程进展, 1999, 19 (5) :72-75. [15] 金世梅, 赵志红, 朱慧, 等.分光光度法检测保健茶中左旋肉碱[J].粮食与食品工业, 2011, 18 (5) :54-57.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
