Study of ultrasonic and microwave synergistic effect on aqueous extraction of Chrysanthemum Morifolium Ramat
-
摘要: 采用超声-微波协同水提杭白菊汁。以总黄酮、绿原酸、固形物的提取率为指标,研究料液比、微波功率、提取时间对杭白菊水提液品质的影响。通过单因素和正交实验确定制备最佳条件:微波功率250W,提取时间240s,料液比1∶30（g∶mL）。此条件下,总黄酮提取率达62.20mg/g,绿原酸提取率达4.05mg/g,固形物提取率50.75%。与常温浸提、热回流、单独超声辅助、单独微波辅助法相比,本实验方法可在较低料液比下得到更高的提取率,且提取时间大大减少。
-
关键词:
- 超声-微波协同萃取（UMAE） /
- 杭白菊水提液 /
- 总黄酮 /
- 绿原酸 /
- 固形物
Abstract: Chrysanthemum Morifolium Ramat ( CMR) was extracted with water by ultrasonic- microwave synergistic method.The effect of solid- liquid ratio, microwave power and extraction time on the extraction yield of total flavonoids, chlorogenic acid and solid content of CMR water extract was studied.The optimal extraction conditions were determined through single-factor and orthogonal experiments as follows: microwave power 250W, extraction time 240s, and solid- liquid ratio 1 ∶ 30. Under above conditions, the extraction yield of total flavonoids reached62.20mg /g, while chlorogenic acid and solid content reached 4.05mg /g and 50.75% respectively. Compared with traditional water extraction methods and lately new methods, ultrasonic or microwave method, the method studied in this paper has the advantages of low solvent usage and high efficiency. -
[1] 喻昌发, 吴东根, 徐立军, 等.浙江省杭白菊品种及其栽培技术应用研究[J].中国果菜, 2011 (6) :39-41. [2] 沈伟桥, 孙海燕, 汪俏梅, 等.杭白菊生物活性成分及药理研究进展[J].茶叶, 2006, 32 (3) :141-144. [3] Choi HS, Kim GH.Volatile Flavor Composition of Gamguk.Chrysanthemum indicum) Flower Essential Oils[J].Food Sci.Biotechnol, 2011, 20 (2) :319-325.
[4] 廖维良, 赵美顺, 杨红.超声波辅助提取技术研究进展[J].广东药学学报, 2012, 289 (3) :347-349. [5] 卢彦芳, 张福成, 安静, 等.微波辅助萃取应用研究进展[J].分析科学学报, 2011, 27 (2) :246-252. [6] 孙爱东, 葛毅强, 蔡同一.天然植物次生代谢产物的提取[J].生物学杂志, 1998, 15 (5) :4-7. [7] Ye Q, Liang YR, Lu JL.Effect of different extracting methods on quality of Chrysanthemum Morifolium Ramat.Infusion[J].Asia Pac J Clin Nutr, 2007, 16 (1) :183-187.
[8] 殷红, 胡永洲, 杨鑫骥, 等.正交设计研究杭白菊总提取物及总黄酮提取工艺[J].中国中药杂志, 2004, 29 (8) :737. [9] 曾元儿.中国药典化学成分析简明手册[M].广州:中山大学出版社, 2010.350. [10] 李宗, 陈在敏, 廖雷生, 等.菊花中绿原酸的含量测定[J].中国中药杂志, 1999, 24 (6) :329-330. [11] Sun QL, Hua S, Ye JH, et al.Flavonoids and volatiles in Chrysanthemum morifolium Ramat flower from Tongxiang County in China[J].African Journal of Biotechnology, 2010, 9 (25) :3817-3821.
[12] Yang JH, Di QQ, Zhao J.Effect of microwave irradiation on chemical profile of extracts during the extraction of organic compounds from Astragalus slices[J].Asia-pacific Journal of Chemical Engineering, 2012, 7:418-426.
[13] Ma YQ, Chen JC, Liu H, et al.Effect of Ultrasonic Treatment on the Total Phenolic and Antioxidant Activity of Extracts from Citrus Peel[J].Toxicology and Chemical Food Safety, 2008, 73 (8) :115-120.
[14] 徐菊茂.杭白菊中黄酮类成分的提取动力学研究[J].中国药业, 2012, 21 (16) :31-32. [15] 张志伟, 张双灵, 高振红.微波辅助菊花茶饮料的研制[J].农产品加工·学刊, 2007, 118 (11) :57-58. [16] 叶倩.绿茶和菊花茶饮料色泽褐变机理和控制技术研究[D].杭州:浙江大学, 2008.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
