Anti-oxidative and pro-oxidative character study of lutein
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摘要: 以叶黄素为受试物,采用抗氧化能力评价化学方法,包括氧自由基吸收能力法（ORAC方法）、Trolox Equivalent Antioxidant Capacity Assay（TEAC法）、Folin-Ciocalteu法（FCR实验法）、1,1-二苯基-2-三硝基苯肼法（DPPH法）,同时采用评价生物大分子损伤的脱细胞彗星实验,对叶黄素的抗氧化-促氧化作用进行探索。结果表明,ORAC法和DPPH法表明不同浓度叶黄素分别呈现出抗氧化和促氧化的双重作用;TEAC法表明叶黄素具有抗氧化作用、FCR实验表明叶黄素含有酚类物质较少,酚类物质不是叶黄素抗氧化作用的主要因素;脱细胞彗星实验表明高剂量叶黄素对DNA分子具有损伤。因此,叶黄素在不同浓度表现出抗氧化—促氧化的双重作用。Abstract: In the study, lutein was used as the test material, chemical methods including Oxygen Radical Absorbance Capacity ( ORAC) , Trolox Equivalent Anti- oxidant Capacity assay ( TEAC) , Folin- Ciocalteu Reaction ( FCR) , 1, 1-Diphenyl-2-picrylhydrazylradical2, 2-Diphenyl-1- ( 2, 4, 6-trinitrophenyl) hydrazyl ( DPPH) were used to evaluate the anti- oxidative character of lutein, and at the same time, the method of evaluating biological macromolecular injury- acellular comet assay was applied in this study.Different concentrations of lutein was demonstrated to have anti-oxidation and pro- oxidation in the ORAC and DPPH; lutein was proved to have antioxidation in TEAC; lutein was indicated to contain less phenolics, and the phenolics was not the main factor of anti-oxidant effect in FCR; high concentration of lutein had damage to DNA in the acellular comet assay.Therefore, lutein showed double effect of anti-oxidant and pro-oxidant in different concentration.
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Keywords:
- lutein /
- anti-oxidant /
- pro-oxidation /
- chemical method /
- acellular comet assay
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