Research advances in hydrolysis of starch by Neopullulannase and its application
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摘要: 新普鲁兰酶（neopullulanase,nPU,EC3.2.1.135）是一种多功能复合型的淀粉水解酶。本文介绍了新普鲁兰酶的结构与性质以及在淀粉水解和转糖苷反应中的作用机理,阐述了新普鲁兰酶在α-淀粉水解酶族13中的分类地位及其在食品工业中的应用,并对其重要的研究价值和应用前景进行了展望。Abstract: Neopullulanase ( nPU, EC 3.2.1.135) is one of the multifunctional complex type of amylase.Firstly, this review discussed the structure and properties of neopullulanase as well as the mechanism in starch hydrolysis and transglycosylation.Secondly, the taxonomic status of neopullulanase in α-amylase family 13 and its adhibition in food industry are elaborated.Finally, its important value of research and the prospect of application were forecasted.
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Keywords:
- Neopullulanase /
- hydrolysis of starch /
- transglycosylation
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[1] Reddy N S, Nimmagadda A, Sambasiva Rao K R S.An overview of the microbialα-amylase family[J].African Journal of Biotechnology, 2003, 2 (12) :645-648.
[2] Majzlova K, Pukajova Z, Janecek S.Tracing the evolution of the alpha-amylase subfamily GH13_36 covering the amylolytic enzymes intermediate between oligo-1, 6-glucosidases and neopullulanases[J].Carbohydr Res, 2013, 367:48-57.
[3] Takata H, Kuriki T, Okada S, et al.Action of neopullulanase.Neopullulanase catalyzes both hydrolysis and transglycosylation atalpha- (1, 4) -and alpha- (1, 6) -glucosidic linkages[J].J.Biol.Chem, 1992, 267 (26) :18447-18452.
[4] Singh R S, Saini G K, Kennedy J F.Covalent immobilization and thermodynamic characterization of pullulanase for the hydrolysis of pullulan in batch system[J].Carbohydr Polym, 2010, 81 (2) :252-259.
[5] Zareian S, Khajeh K.Purification and characterization of a novel amylopullulanase that converts pullulan to glucose, maltose, and maltotri-ose and starch to glucose and maltose[J].Enzyme Microb Technol, 2010, 46:57-63.
[6] Hondoh H, Kuriki T, Matsuura Y.Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase[J].J Mol Biol, 2003, 326 (1) :177-188.
[7] Kamitori S, Abe A, Ohtaki A, et al.Crystal structures and structural comparis on of Thermoactinomyces vulgaris R-47α-amylaseⅠ (TVAⅠ) at 116A resolution andα-amylaseⅡ (TVAⅡ) at 213A resolution[J].J Mol Biol, 2002, 318 (2) :443-453.
[8] Mizuno M, T onozuka T, Uechi A, et al.The crystal structure of Thermoactinomyces vulgaris R-47α-amylaseⅡ (TVAⅡ) complexed with transglycosylated product[J].Eur J Biochem, 2004, 271 (12) :2530-2538.
[9] Yoon S A, Soo-In R, Soo-Bok L, et al.Purification and Characterization of Branching Specificity of a Novel Extracellular Amylolytic Enzyme from Marine Hyperthermophilic Rhodothermus marinus[J].J Microbiol Biotechnol, 2008, (3) :457-464.
[10] Saburi W, Morimoto N, Mukai A.A thermophilic alkalophilic alpha-amylase from Bacillus sp.AAH-31 shows a novel domain organization among glycoside hydrolase family 13 enzymes[J].Biosci Biotechnol Biochem, 2013, 77 (9) :1867-1873.
[11] Maria J Y, Jaime A, Pascual S, et al.Characterization of Novel Neopullulanase from Bacillus polymyxa[J].Appl Biochemistry and Biotechnology, 1997, 68:113-120.
[12] Jutta M, Anja H, Erwin S, et al.Gene cloning, nucleotide sequence and biochemical properties of a cytoplasmic cyclomaltodextrinases (neopullulanase) from Alicyclobacillus acidocaldarius, reclassi￠cation of a group of enzymes[J].FEMS Microbiology Letters, 2000, 183:55-61.
[13] 王阳, 李樊, 张应玖.α-淀粉酶家族的新成员—多功能淀粉酶[J].中国生物化学与分子生物学报, 2009, 25 (2) :104-109. [14] Kamasaka H, Sugimoto K, Takata H.Bacillus stearothermophilus neopullulanase selective hydrolysis of amylose to maltose in the presence of amylopectin[J].Appl Environ Microbiol, 2002, 68 (4) :1658-1664.
[15] Yebra M J, Arroyo J, Sanz P, et al.Characterization of novel neopullulanase from Bacillus polymyxa[J].Appl.Biochem.Biotechnol, 1997, 68:113-120.
[16] Kamakshi P, Rajdevi, Yogeeswaran G.Cooperativity and Substrate Specificity of an Alkaline Amylase and neopullulanase complex of micrococcus halobius OR-1[J].Applied Biochemistry and Biotechnology, 2001, 90:222-249.
[17] Tonozuka T, Ohtsuka M.A neopullulanase-typeα-amylase gene from Thermoactinomyces vulgaris R-47[J].Biosci Biotechnol Biochem, 1993, 57:395-401.
[18] Smith K A, Salyers A A.Characterization of a neopullulanase and aα-glucosidase from Bacteroides thetaiotaomicron 95-1[J].J Bacteriol, 1991, 173:2962-2968.
[19] Fogarty W M, Kelly C T.Recent advances in microbial amylases.In microbial enzymes and biotechnology[J].Elsevier, London, 1990, 71-132.
[20] Igarashi K, Ara K, Saeki K, et al.Nucleotide sequence of the gene that encodes a neopullulanase from alkalophilic Bacillus[J].Biosci Biotechnol Biochem, 1992, 56:514-516.
[21] Kadziola A, Ji A, Svensson B, et al.Crystal and molecular structure of barleyα-amylase[J].J Mol Biol, 1994, 217:737-750.
[22] Nakada T, Kubota M, Sakai S, et al.Purification and characterization of two forms of maltotetraose-forming amylase from Pseudomonas stutzeri[J].Agric Biol Chem, 1990, 54:737-743.
[23] Ohdan K, Kuriki T, Kaneko H, et al.Characterization of two formα-amylase and structure implication[J].Appl Environ Microbiol, 1999, 65:4652-4658.
[24] Takehiro Y, Takashi T, Shigehiro K, et al.The Deletion of Amino-Terminal Domain in Thermoactinomyces vulgaris R-47α-Amylases:Effects of domain N on Activity, Specificity, Stability and Dimerization[J].Biosci Biotechnol Biochem, 2001, 65:401-408.
[25] Vikash K.Analysis of the key active subsites of glycoside hydrolase 13 family members[J].Carbohyd Res, 2010, 345:893-898.
[26] 王盼盼.食品食品加工中的增稠剂 (七) 食品增稠剂——淀粉、变性淀粉及淀粉水解物[J].肉类研究, 2010, 134 (4) :47-54. [27] 徐正康, 黄立新.中国淀粉水解工业发展及其展望思考[J].现代食品科技, 2009, 25 (8) :855-859. [28] 孙晓晨, 初晓宇, 伍宁丰.细菌I型普鲁兰酶的研究进展[J].中国农业科技导报, 2012, 14 (2) :73-80. [29] 袁美兰.淀粉结构和性质的研究概况[J].畜牧与饲料科学, 2011, 32 (1) :102-104. [30] 叶红玲, 杜先锋.全酶法制备超高麦芽糖浆工艺[J].食品科学, 2010, 31 (20) :15-19. [31] 林亲录, 符琼, 周丽君.低聚异麦芽糖制备的研究进展[J].食品工业科技, 2011, 32 (2) :398-400. [32] Park KH, KimT J, CheongTK, et al.Structure, specificity and function of cyclomaltodextrinase, a multispecific enzyme of theα-amylase family[J].Biochim Biophy Acta, 2000, 1478 (2) :165-185.
[33] Balcerek M, Pielech-Przybylska K.Effect of supportive enzymes on chemical composition and viscosity of rye mashes obtained by the pressureless liberation of starch method and efficiency of their fermentation[J].Eur Food Res Technol, 2009, 229:141-151.
[34] Zhang H X, Jin Z Y.Preparation of resistant starch by hydrolysis of maize starch with pullulanase[J].Carbohyd Polymers, 2011, 83:865-867.
[35] Park K H.Function and tertiary-and quaternary-structure of cyclodextrin-hydrolyzing enzymes (CDase) , a group of multisubstrate specific enzymes belonging to theα-amylase family[J].J Appl Glycosci, 2006, 53 (1) :35-44.
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