Investigation of deproteinization technology for polysaccharide from gel of Aloe vero
-
摘要: 为研究库拉索芦荟凝胶多糖蛋白质的脱除工艺条件,本文以蛋白质脱除率和多糖保留率为指标,比较三氯乙酸（TCA）法、Sevag法、木瓜蛋白酶法、木瓜蛋白酶-TCA联用法、木瓜蛋白酶-Sevag联用法脱除芦荟凝胶多糖蛋白的效果。结果显示,木瓜蛋白酶-TCA联用法效果最好,其条件为酶用量2%、酶解温度60℃、酶解时间1h、pH为6,采用5%TCA除蛋白1次,此时蛋白质去除率为83.4%,多糖保留率为74.9%。不同脱蛋白法对芦荟凝胶多糖的脱蛋白效果有较明显差异,木瓜蛋白酶-TCA联用法是一种有效的芦荟凝胶多糖脱蛋白方法。Abstract: To study the deproteinization technology of polysaccharide from gel of Aloe vero, the deproteinization rate and the retention rate of polysaccharide were as comprehensive evaluation index, deproteinization effect of five methods including TCA, Sevag, papain, papain-TCA, papain-Sevag on polysaccharide from gel of Aloe vero were compared. Results showed that papain- TCA method was the optimum deproteinization technology and its operation was: the amount of papain 2%, enzymatic time 1h, temperature 60℃, pH 6 and followed by one treatment with 5% TCA. Under this condition, the percentage of deproteinization was 83.4%, and the retention of polysaccharide was 74.9%. Deproteinization effect on polysaccharide from gel of Aloe vero had significant difference by different method, papain- TCA was an effective method for removal of protein from gel polysaccharide of Aloe vero.
-
Keywords:
- Aloe vero /
- gel polysaccharide /
- removal of protein
-
[1] 刘小平, 卢金利, 吴素歌, 等.芦荟凝胶对大鼠Ⅱ度放射性皮炎创面愈合及表皮生长因子、碱性成纤维细胞生长因子表达的影响[J].西安交通大学学报:医学版, 2006, 27 (1) :66-68. [2] 李国豪, 雷秀霞, 徐邦牢, 等.芦荟大黄素对肺炎链球菌侵袭A549细胞的抑制作用[J].热带医学杂志, 2005, 5 (3) :276-278. [3] 田兵, 华跃进, 马小琼, 等.芦荟抗菌作用与蒽醌化合物的关系[J].中国中药杂志, 2003, 28 (11) :1034-1037. [4] 国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社, 1999:461. [5] Zhang L, Tizard IR.Activation of a mouse macrophage cell line byacemannan:the major carbohydrate fraction from Aloe vera gel[J].Immunopharmacol, 1996, 35 (2) :119.
[6] 王健, 龚兴国.多糖的抗肿瘤及免疫调节进展[J].中国生化药物杂志, 2001, 22 (1) :52-54. [7] 国家药典委员会.中国药典2005版[S].北京:化学工业出版社, 2005. [8] 邢小黑, 吴明忠, 朱述钧, 等.灵芝多糖化学研究[J].中国食用菌, 1996 (3) :14-16. [9] 伍善广, 赖泰君, 孙建华, 等.蚕蛹多糖脱蛋白方法研究[J].食品科学, 2011 (14) :21-24. [10] 李燕, 王晓丽, 俞飞锋.响应曲面法优化超声辅助提取芦荟凝胶多糖的工艺[J].食品研究与开发, 2010 (6) :17-21. [11] 张雅利, 张文娟, 何琼华.胰蛋白酶-Sevag法联用脱柿多糖蛋白[J].食品工业科技, 2005 (5) :107-108. [12] 李强, 唐微, 郑伟, 等.杜仲粗多糖脱蛋白方法的对比研究[J].食品工业科技, 2011 (3) :316-317. [13] 王传名, 董祺, 管从胜.日照绿茶粗多糖脱蛋白研究[J].食品工业科技, 2010 (8) :274-276.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
