Separation and purification process of total flavonoids from Sedum aizoon leaves
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摘要: 本文采用了AB-8大孔吸附树脂对费菜总黄酮进行分离纯化,利用静态和动态吸附洗脱结合的方法,以AB-8大孔树脂的吸附率和解析率为指标考察了pH、洗脱剂浓度、洗脱时间、吸附流速、洗脱流速、洗脱剂用量、洗脱蒸馏水用量等对费菜总黄酮的分离纯化效果及影响因素。结果表明费菜总黄酮分离纯化的适宜工艺条件:吸附液pH为5.0、洗脱剂为80%乙醇、洗脱时间24h、吸附流速12mL/min、洗脱流速2mL/min、洗脱剂用量80mL、洗脱蒸馏水用量100mL。在该条件下,AB-8大孔树脂的饱和吸附量为11mg/g、静态吸附率68.3%、解析率94.5%、费菜总黄酮纯度47.98%。Abstract: A method for the purification of total flavonoids from Sedum aizoon with AB-8 types of macroreticular adsorbents was studied by using combining static adsorption with elution and dynamic adsorption with elution.The adsorption and desorption rate of AB- 8 types of macroreticular adsorbents were calculated to determine the effectiveness, and influence factors of separation and purification of total flavonoids with the effects of pH value, elution ethanol concentration, elution time, the adsorption current velocity, the desorption current velocity, the eluant volume, the distilled water of elution valume. Its optimum separation and purification process conditions were determined as follows: the pH value of extracts was 5.0, 80% alcohol as the eluent, 24 h as eution time, 80 mL volume as the eluant volume and 100 mL volume as the distilled water of elution, the adsorption current velocity as 1~2mL/min and the desorption current velocity as 2mL /min.Under the conditions, the static adsorption capacity of AB-8 type of macroteticular adsorbent was 11 mg /g and the static adsorption rate was 68.3% while the desorption rate was94.5%.
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