Study on isolation, structural characterization and antioxidant activity evaluation of polysaccharide PP60-S1 from Phellinus pini
-
摘要: 松木层孔菌子实体水提物经乙醇分级醇沉和DEAE-Sepharose Fast Flow离子柱层析及Sephacryl S-300凝胶柱层析纯化,获得均一多糖PP60-S1,其分子量为3.56×104u,是由葡萄糖、甘露糖和半乳糖组成的杂多糖,单糖摩尔比为9.24∶1.00∶0.76,甲基化分析PP60-S1主要由1-Glc、1,3-Glc、1,6-Glc、1,3-Gal、1,6-Gal、1,3,6-Man 6种糖苷键连接方式,主链主要由1,6-Glc构成。PP60-S1的体外抗氧化活性研究表明其具有一定的DPPH自由基和ABTS+自由基清除活性以及FRAP总抗氧化能力。Abstract: The homogeneous polysaccharide PP60-S1 was obtained by water extraction, ethanol precipitation and purified by DEAE-Sepharose F. F. and Sephacryl S-300 from the fruiting bodies of Phellinus pini. The polysaccharide PP60-S1 was composed of glucose, mannose and galactose with a molar ratio of 9.24∶1.00∶0.76, with an average molecular weight of 3.56×104u. The results of methylation analysis indicated that six kinds of glycosidic linkages were detected in the structure of PP60-S1:1-Glc、1, 3-Glc、1, 6-Glc、1, 3-Gal、1, 6-Gal、1, 3, 6-Man, respectively. The main chain contained mainly 1, 6-Glc. Otherwise, the polysaccharide PP60-S1 exhibited a certain degree of antioxidant activity, evaluated by in vitro assay of DPPH·, ABTS+· and ferricreducing antioxidant power (FRAP) .
-
Keywords:
- Phellinus pini /
- polysaccharide /
- purification /
- structural characterization /
- antioxidant activity
-
[1] Jang H J, Yang K S.Inhibition of nitric oxide production in RAW 264.7 macrophages by diterpenoids from Phellinus pini[J].Archives of Pharmacal Research, 2011, 34 (6) :913-917.
[2] William A A, David J M, Priyotosh C.Phenolic and other metabolites of Phellinus pi ni, a fungus pathogenic to pine[J].Phytochemistry, 1996, 42 (5) :1321-1342.
[3] 王稳航, 李玉刘, 安军.松木层孔菌多糖的提取及抗氧化性研究[J].食品研究与开发, 2006, 27 (11) :53-55. [4] 王稳航, 李玉, 刘安军.松木层孔菌多糖对正常小鼠体内抗氧化功能的影响[J].现代生物医学进展, 2008, 8 (8) :1439-1441. [5] 裴丽娟, 袁蕾, 马姣妮, 等.松木层孔菌多糖PEP的结构及活性研究[J].分子科学学报, 2010, 26 (2) :103-107. [6] 袁蕾, 刘瑜, 田法益, 等.松木层孔菌 (Phellinus pini) 胞外多糖结构解析[J].中国药学杂志, 2013, 48 (8) :597-600. [7] 刘安军, 任寅, 王稳航, 等.水溶性Phellinus pini多糖PS1的分离提取及免疫活性研究[J].现代生物医学进展, 2006, 6 (11) :14-17. [8] Lee S M, Kim S M, Lee Y H, et al.Macromolecules isolated from Phellinus pini fruiting body:Chemical characterization and antiviral activity[J].Macromolecular Research, 2010, 18 (6) :602-609.
[9] Milardovic S, Ivekovic D, Grabaric B S.A novel amperometric method for antioxidant activity determination using DPPH free radical[J].Bioelectrochemistry, 2006, 68 (2) :175-180.
[10] Miller N J, Rice E C, Davies M J, et al.A novel method for measuring antioxidant status in premature neonates[J].Clinical Science, 1993, 84 (4) :407-412.
[11] Benzie I F F, Strain J J.The ferric reducing ability of plasma (FRAP) as a measure of“antioxidant power”:the FRAP assay[J].Analytical Biochemistry, 1996, 239 (1) :70-76.
[12] Luo J G, Liu J, Ke C L, et al.Optimization of medium composition for the production of exopolysaccharides from Phellinus baumii Pilát in submerged culture and the immunostimulating activity of exopolysaccharides[J].Carbohydrate Polymers, 2009, 78:409-415.
[13] Bao X F, Duan J Y, Fang X, et al.Chemical modifications of the (1→3) -alpha-D-glucan from spores of Ganoderma lucidum and investigation of their physciochemical properties and immunological activity[J].Carbohydrate Research, 2001, 336:127-140.
[14] Yang B, Jiang Y M, Zhao M M, et al.Structural characterisation of polysaccharides purified from longan (Dimocarpus longan Lour.) fruit pericarp[J].Food Chemistry, 2009, 115:609-614.
[15] Zhang A Q, Xiao N N, Deng Y L, et al.Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes[J].Carbohydrate Polymers, 2012, 87:2279-2283.
[16] Kondakova A N, Fudala R, Senchenkova S N, et al.Structure of a lactic acid ether-containing and glycerol phosphate-containing O-polysaccharide from Proteus mirabilis O40[J].Carbohydrate Research, 2005, 340:1612-1617.
[17] Fan J M, Zhang J S, Tang Q J, et al.Structural elucidation of a neutral fucogalactan from the mycelium of Coprinus comatus[J].Carbohydrate Research, 2006, 341:1130-1134.
[18] Bao X F, Liu C P, Fang J N, et al.Structural and immunological studies of a major polysaccharide from spores of Ganoderma lucidum (Fr.) Karst[J].Carbohydrate Research, 2001, 332:67-74.
[19] Kocharova N A, Ovchinnikova O G, Toukach F V, et al.The O-polysaccharide from the lipopolysaccharide of Providencia stuartii O44 contains L-quinovose, a 6-deoxy sugar rarely occurring in bacterial polysaccharides[J].Carbohydrate Research, 2005, 340:1419-1423.
[20] 杨开, 薛介丰, 金月忠, 等.松木层孔菌多糖的微波提取和体外活性研究[J].食药用菌, 2013, 21 (2) :102-107. [21] Chen H X, Zhang M, Qu Z S, et al.Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis) [J].Food Chemistry, 2008, 106:559-563.
[22] Huang S Q, Ding S D, Fan L P.Antioxidant activities of five polysaccharides from Inonotus obliquus[J].International Journal of Biological Macromolecules, 2012, 50:183-1187.
[23] Ma L S, Chen H X, Zhang Y, et al.Chemical modification and antioxidant activities of polysaccharide from mushroom Inonotus obliquus[J].Carbohydrate Polymers, 2012, 89 (2) :371-378.
[24] Zhang Y L, Zhang J B, Mo X Y, et al.Modification, characterization and structure-anticoagulant activity relationships of persimmon polysaccharides[J].Carbohydrate Polymers, 2010, 82 (2) :515-520.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
