Study on the antioxidant activity of different extracts from Rubia tibetica Hook.F.
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摘要: 采用DPPH自由基清除法、ABTS自由基清除法、羟自由基清除法、超氧阴离子清除法、还原力测定法和螯合力测定法六种抗氧化模型对藏茜草95%乙醇提取物以及石油醚相,乙酸乙酯相,正丁醇相和水相等4个不同极性部位的抗氧化活性进行评价,同时分析抗氧化活性与总酚和总黄酮含量的关系。研究结果表明,除水提部位外,藏茜草其它4个极性部位提取物均表现出一定的抗氧化活性,其抗氧化活性与多酚和总黄酮含量呈显著相关。其中,乙酸乙酯部位总黄酮和总多酚含量最高,抗氧化活性也最强,其总黄酮和总多酚含量分别为(232.03±1.74)mg芦丁当量/g提取物和(173.53±1.75)mg没食子酸当量/g提取物,其清除DPPH自由基、超氧阴离子、羟自由基和ABTS自由基的EC50分别为0.06±0.01、0.17±0.01、(0.24±0.02)mg/m L和(1.75±0.23)μg/m L,对金属离子螯合力的EC50为(0.11±0.01)mg/m L。藏茜草的乙酸乙酯极性部位具有显著的抗氧化活性,是天然抗氧化活性化合物的良好来源。Abstract: The present study was carried out to evaluate the antioxidant capacity of the 95% ethanol crude extracts and their four solvent fractions( petroleum ether,ethyl acetate,normal butyl alcohol and aqueous) of Rubia tibetica Hook.F. The extracts of the Rubia tibetica Hook. F. were evaluated for their total phenols,total flavonoids and antioxidant activity( DPPH radical,ABTS radical,hydroxyl radical,superoxide scavenging effect,reducing power and ferrous ions chelating activities). The relationship between the antioxidant activity and the content of total phenolics and total flavonoids was analysed.The results showed that all the fraction except water fraction had great antioxidant activity and the content of total phenolics and total flavonoids had a highly significant correlation with the activity.The ethyl acetate fraction was the most effective fraction with the highest content of total phenolics and total flavonoids and the content of them were( 232.03 ± 1.74) mg rutin equivalent / g extract and( 173.53 ± 1.75) gallic acid equivalent mg / g extract,respectively. The EC50 values of ethyl acetate fraction based on DPPH,superoxide,hydroxyl radical and ABTS was 0.06 ± 0.01,0.17 ± 0.01,( 0.24 ± 0.02) mg / m L and( 1.75 ± 0.23) μg / m L. The EC50 of ethyl acetate fraction on ferrous ions chelating activities was( 0.11 ± 0.01) mg / m L. Ethyl acetate fraction of Rubia tibetica Hook.F,a strong ability to act as antioxidant,may be considered as a natural source of active compounds.
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Keywords:
- Rubia tibetica Hook.F /
- antioxidant activity /
- total flavonoids /
- total phenolics
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