Simultaneous determination of calcium propionate and natamycin in cake by high performance liquid chromatography with programmed wavelength UV detection
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摘要: 建立一种同时测定糕点中丙酸钙和纳他霉素的变波长梯度洗脱高效液相色谱检测方法。样品采用超声处理,以甲醇和0.01mol/m L酸性磷酸氢二铵为流动相进行梯度洗脱,经色谱柱为C18(4.6mm×150mm,5μm)分离,程序化变波长检测,外标法定量。该方法可以将丙酸钙和纳他霉素两种防腐剂很好的分离,保留时间分别为(3.5±0.2)min和(9.4±0.2)min,丙酸钙和纳他霉素的线性回归方程相关系数均大于0.9999,方法回收率为89%~98%。采用高效液相色谱同步测定糕点中丙酸钙和纳他霉素的方法具有快速、准确、灵敏度和精密度高的优点,能满足糕点中丙酸钙和纳他霉素含量同步测定的要求。Abstract: A method of simultaneous determination of calcium propionate and natamycin in cake by high performance liquid chromatography with programmed wavelength UV detection was developed.The samples were extracted with ultrasound pretreatment,separated with Agilent Zorbax SB- C18( 4.6mm × 150mm) using methanol-di- ammonium hydrogen phosphate( 0.01 mol / m L) as mobile phase with a gradient elution program,determined with variable wavelength detector,and quantified with external standard curve. This method could achieve the separation of calcium propionate and natamycin in cake,and the retention time of calcium propionate and natamycin was( 3.5 ± 0.2) min and( 9.4 ± 0.2) min,respectively.The correlation coefficients of calibration curves were all above 0.9999,and average recoveries were 89% ~ 98%.It could conclude that the method that using HPLC to simultaneously determine calcium propionate and natamycin in cake had characters of quickness,accuracy and high- sensitivity,which could meet the requirements of simultaneously determining calcium propionate and natamycin in cake.
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Keywords:
- HPLC /
- cake /
- calcium propionate /
- natamycin
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