Comparation on synthesis and release of mannan in four yeasts
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摘要: 比较了产朊假丝酵母、毕赤酵母、面包酵母和啤酒酵母培养过程中细胞壁甘露聚糖的合成与释放特性。结果表明:四种酵母的甘露聚糖合成均为生长偶联型;不同类型酵母的甘露聚糖合成能力差异显著(p<0.05),合成能力最强的啤酒酵母细胞壁甘露聚糖含量最高可达0.62g/L发酵液,而合成能力最弱的毕赤酵母仅为0.47g/L发酵液。四种酵母的甘露聚糖最大释放量均出现对数生长中期,并且不同类型酵母释放效率差异较大,最高值毕赤酵母甘露聚糖释放量达到了0.27g/L发酵液,最低值啤酒酵母仅有0.15g/L发酵液。乙醇显著刺激四种酵母的甘露聚糖释放(p<0.05),其中对产朊假丝酵母的影响最显著且在5%乙醇浓度下,与对照相比,其甘露聚糖释放量和释放率分别大幅提高了152%和120%。Abstract: The synthesis and release rules of mannan on cell walls was compared during the cultivation process of candida utilis, pichia pastoris, bread yeast and beer yeast in this research. It was found that the syntheses of mannan in these four yeasts were all growth coupling type. Synthetic ability of mannan in different types of yeast were significantly different ( p < 0. 05) . The synthetic ability of mannan in beer yeast, which had the strongest synthetic ability among these four yeasts, was 0.62g/L fermentation broth, while that in pichia pastoris was only 0.47g/L fermentation broth. It was indicated that all the maximal releasing rates of mannan in four kinds of yeasts appeared in the middle period of logarithmic growth and the maximal releasing rates of mannan in different types of yeasts differed significantly, i.e., the maximal releasing amount of mannan in pichia pastoris, which was highest among these four kinds of yeasts, reached to 0.27g/L fermentation broth, while the maximal releasing amount of manna in beer yeast, which was the lowest among these four kinds of yeasts, was only 0.15g/L fermentation broth. It was showed that alcohol significantly stimulated the releasing of mannan in four kinds of yeasts ( p < 0. 05) . Candida utilis in the alcohol with a concentration of 5 % was affected most significantly. The releasing amount and releasing rate of mannan in it were respectively increased by 152% and120% as compared with the control (p<0.05) .
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[1] 段胜林, 王雪, 苑鹏, 等.采用催化自溶和生物破壁技术提取啤酒酵母细胞壁多糖[J].食品与发酵工业, 2012, 38 (5) :138-144. [2] 汪立平, 王锡昌, 陈有容, 等.啤酒废酵母甘露聚糖的制备[J].食品科学, 2009, 30 (2) :134-137. [3] 刘红芝.酿酒酵母甘露聚糖的制备、结构鉴定及免疫活性研究[D].北京:中国农科院, 2009. [4] 王义华, 徐梅珍, 江萍, 等.酵母蛋白多糖的分离纯化及鉴定[J].微生物学报, 2004 (8) :515-518. [5] 刘红芝, 王强, 周素梅.酵母甘露聚糖分离提取及功能活性研究进展[J].食品科学, 2008, 29 (5) :465-468. [6] 孙建义, 李卫芬.啤酒酵母甘露聚糖的提取及其对鸡肠道微生物区系的影响[J].浙江大学学报:农业与生命科学版, 2001, 27 (4) :447-450. [7] 温若竹.甘露寡糖对肉仔鸡肠道形态及微生物区系的影响[D].南京:南京农业大学, 2010. [8] Shashidhara R G, Devegowda G.Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity[J].Poultry Science, 2003, 82:1319-1325.
[9] Shafey TM, Mufarej AS, Shalaby MI, et al.Effects of mannan oligosaccharides on antibody response to infectious bronchitis, infectious bursal disease and Newcastle disease in chickens[J].J Appl Anim Res, 2001, 19:117-127.
[10] Ponton J, Omaetxebarria J, Elguezabal N, et al.Immunoreactivityof the fungal cell wall[J].Medical Mycology, 2001, 39:101-110.
[11] Palomero F, Morata A, Benito S, et al.Conventional and enzyme-assisted autolysis during ageing over lees in red wines:Influence on the release of polysaccharides from yeast cell walls and on wine monomeric anthocyanin content[J].Food Chemistry, 2007, 105:838-846.
[12] Giovani G, Rosi I.Release of cell wall polysaccharides from Saccharomyces cerevisiae thermosensitive autolytic mutants during alcoholic fermentation[J].International Journal of Food Microbiology, 2007, 116:19-24.
[13] Quirós M, Gonzalez-Ramos D, Tabera L, et al.A new methodology to obtain wine yeast strains overproducing mannoproteins[J].International Journal of Food Microbiology, 2010, 139 (1) , 9-14.
[14] 刘媛媛.酵母细胞壁多糖制备及流变学性质研究[D].北京:中国农科院, 2010. [15] Guilloux-Benatier M, Guerreau J, Feuillat M.Influence of initial colloid content on yeast macromolecule production and on the metabolism of wine microorganisms[J].American Journal of Enology and Viticulture, 1995, 46:486-492.
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