Determination of amylose and amylopectin content in fresh yam by dual- wavelength spectrophotometry
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摘要: 以新鲜怀山药为原料制备山药粉，通过石灰水浸泡工艺提取山药淀粉。以碘为显色剂，扫描直链淀粉和支链淀粉与碘形成复合物的全波长吸收光谱。利用不同浓度的直链与支链淀粉标准品分别与碘形成复合物从而得到淀粉含量与吸光度差值之间的线性关系制作出标准曲线，根据标准曲线计算出样品中直链淀粉与支链淀粉的含量。根据双波长选择原理和扫描光图谱，确定直链淀粉的测定波长为 600nm，参比波长为 532nm; 支链淀粉的测定波长为560nm，参比波长 659nm。实验说明直链淀粉与支链淀粉的测定相对标准偏差分别为 0.27% 与 0.63% 、回收率也分别达到了96.18%与97.89%，检出限分别为0.09mg/L 与 1.58mg/L，再经准确性分析验证了用双波长法测定山药淀粉中直链淀粉与支链淀粉的含量的检测方法具有较为准确的效果。Abstract: Yam starch was extracted through soak process by whitewash with yam powder which was from fresh yam as raw material.A standard curve was got based on the line relationship between absorption difference and content of starch,which the concentration cycle of standard amylose and amylopectin were used. This research had finally got 600 nm for measure wavelength while 560 nm for reference wavelength of amylase,532 nm for measure wavelength while 644 nm for reference wavelength of amylopectin according to the amylose and amylopectin complex full wavelength absorption spectra with iodine as chromogenic agent. The research about content of amylose and amylopectin in the sample with the standard curve had been worked out.According to the experiment,the RSD were 0.27% and 0.63% respectively,recovery data were 96.18% and 97.89%,while detection limit were 0.09 mg / L and 1.58 mg / L based on the amylose and amylopectin test. Appending accuracy analysis,allconfirmed that the dual- wavelength determining the content of amylose and amylopectin had more accurate effect.
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Keywords:
- yam /
- dual-wavelength /
- amylose / amylopectin
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