Optimization of the condition of decoloring process of Huidouba polypeptide by response surface methodology
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摘要: 本文以灰兜巴蛋白的酶解液为原料,首次采用粉末活性炭对多肽液进行脱色处理,去除灰兜巴多肽液中的色素,以利于生物活性多肽的分离纯化。在单因素实验的基础上,用响应曲面法优化灰兜巴酶解液的脱色工艺。实验结果表明脱色剂为粉末活性炭时脱色效果最佳,其工艺参数为:p H3.3、活性炭用量1.6%、温度44℃、时间53min。在此条件下,灰兜巴蛋白酶解液的脱色率为73.12%,肽回收率为82.49%,与理论值相接近。该脱色工艺简单可靠,脱色效果好,且最大限度的保留了灰兜巴蛋白酶解液中的多肽含量,为开发灰兜巴多肽提供理论基础。Abstract: Huidouba polypeptide from Huidouba protease hydrolyzate had been decolored by activated carbon powder.It was helpful to separate and purify the bioactive peptides by removing the pigment from Huidouba polypeptide hydrolyzate.Based on the results of single factor experiments, the decoloring process was optimized by response surface methodology. Activated carbon powder was the best as a bleaching agent and the optimal conditions were as follows: p H3.3, The amount of activated carbon 1.6%, temperature 44℃, time 53 min. Under the optimal conditions, the decoloring rate was 73.12%, and the peptide retention rate was 82.49% which were close to theoretical value. The decoloring process was cost- effective, simple, reliable, and maximized the retention of Huidouba protease hydrolyzate with high peptide retention rate.The results would provide a theoretical foundation for the development of Huidouba polypeptide.
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