Optimization of extraction conditions of pulsed light parameters for enhanced sterilization of mould on pasteurized milk by response surface methodology
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摘要: 为研究脉冲强光杀菌技术对巴氏奶杀菌的效果,以巴氏奶为研究对象,用霉菌灭菌率作为衡量脉冲强光灭菌技术的指标。在单因素实验基础上,选取闪照次数、闪照距离、闪照能量和样液厚度为自变量,霉菌灭菌率为响应值,利用Box-Benhnken中心组合设计原理和响应面分析法,确定最佳条件为闪照次数35次、闪照距离12.8cm、闪照能量300J、样液厚度2mm。在此条件下,霉菌灭菌率达到99.97%。借助电子鼻测定分析,通过线性判别分析法（LDA）进行气味信息的分析和判断,利用Alphasoft分析软件对奶样进行货架期预测,得到空白样和对照样的预测货架期天数分别是5d和7d,说明脉冲强光灭菌技术可以有效延长巴氏奶的货架期。Abstract: To study the sterilization effect of the pulsed light sterilization technology to the pasteurized milk, pasteurized milk as the research object, with the sterilization rate of mould as a measure indicator.Four extraction parameters including flash times, flash distance, flash energy and samples liquid thickness were optimized using central composite design and response surface methodology based on single factor investigation for achieving maximum the sterilization rate of mould. Using Box- benhnken central composite design and response surface analysis theory, the optimum extraction condition for flash times of 20, flash distance of 12.8cm, flash energy of 300 J and samples liquid thickness of 2mm.Under these conditions, the sterilization rate of mould was 99.97%.By means of electronic nose analysis and determination.Odor information of two milk sample were analyzied and judged by linear discriminant analysis ( LDA) .Using the Alphasoft software, it was found that the shelf life of milk sample were5 d and 7d before and after pulsed light treatment. Plused light sterilization technology can extend the shelf life of pasteurized milk effectively.
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Keywords:
- pasteurized milk /
- sterilization /
- shelf life /
- electronic nose /
- pulsed light
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