Preparation of yak pancreatin and its application in lactoalbumin hydrolysate
-
摘要: 基于牦牛胰脏提取胰酶,以酶活力和收率作为衡量指标,在单因素实验的基础上通过正交实验,确定牦牛胰酶制备的最佳工艺,最后探讨胰酶水解酪蛋白制备水解乳蛋白的效果。结果表明,牦牛胰酶最佳制备工艺为牦牛胰脏∶25%乙醇(提取剂)=1∶1,提取激活时间为5h,激活p H=6.8,75%乙醇沉淀8h。所得胰酶中胰蛋白酶、胰淀粉酶和胰脂肪酶的酶活力分别为1867、16298和5683 U/g,收率达9.7%;用其在温度40℃,p H7.5,酶与底物比例为1∶5的条件下水解酪蛋白4 h后所得的水解乳蛋白与Hyclone的水解乳蛋白相比较,有19种相同的氨基酸,且氨基酸总含量高于Hyclone的水解乳蛋白,分别为36.111%和27.725%。综上可知,该工艺简单,耗时短,易于产业化,在水解蛋白质领域中具有良好的应用前景。Abstract: Pancreatin was extracted from yak pancreas,enzyme activity and yield was used as the measurement index.On the basis of single factor experiment,the orthogonal test was used to determine the optimization of technological parameters of yak pancreatin preparation,at the same time,the effects of hydrolyzing casein was investigated using pancreatin to prepare lactoalbumin hydrolysate. The results showed that the optimum technological condition for preparation of yak pancreatin was as follows: yak pancreas ∶ 25% ethanol( extracting agent) = 1 ∶ 1,extraction and activation time was 5 h,p H = 6.8,75% ethanol precipitated for 8 h. Under these conditions,enzyme activity of trypsin,amylase and lipase were 1867,16298 U / g and 5683 U / g respectively,the yield reached 9.7%.Then the pancreatin add to casein with ratio of 1∶5 to prepare lactoalbumin hydrolysate under40 ℃,p H7.5 for 4 h. Consistant with Hyclone,there were 19 kinds of amino acids in hydrolysis product,but the contants of total amino acids were higher than Hyclone: the percentage was 36.111% and 27.725% respectively.In conclusion,this technique is simple,time saving,feasibility to be industrialized,and will be a good application prospect in the field of protein hydrolysate.
-
Keywords:
- yak pancreas /
- pancreatin /
- enzymatic activity /
- yield /
- lactoalbumin hydrolysate
-
[1] 中华人民共和国药典委员会.中华人民共和国药典(二部)[M].北京:中国医药科技出版社,2010,848-849. [2] Wu Y,Wang Y J,Luo GS,et al.In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution[J].Bioresource Technology,2009,100(14):3459-3464.
[3] Sikkens E C M,Cahen D L,Kuipers E J,et al.Pancreatic enzyme replacement therapy in chronic pancreatitis[J].Best Practice&Research Clinical Gastroenterology,2010,24(3):337-347.
[4] 魏文毅,张丽萍,王琴,等.猪胰脏中胰酶的制备新工艺技术研究[J].黑龙江八一农垦大学学报,2011,23(1):72-78. [5] Segura-Campos M R,Tovar-Benítez T,Chel-Guerrero L,et al.Functional and bioactive properties of Velvet bean(Mucuna pruriens)protein hydrolysates produced by enzymatic treatments[J].Journal of Food Measurement and Characterization,2014,8(2):61-69.
[6] Demirhan E,Apar D K,zbek B.Sesame cake protein hydrolysis by alcalase:Effects of process parameters on hydrolysis,solubilisation,and enzyme inactivation[J].Korean Journal of Chemical Engineering,2011,28(1):195-202.
[7] Yarnpakdee S,Benjakul S,Kristinsson H G,et al.Antioxidant and sensory properties of protein hydrolysate derived from Nile Ti-lapia(Oreochromis niloticus)by one-and two-step hydrolysis[J].Journal of Food Science and Technology,2014:1-14.
[8] Montoya-Rodríguez A,Milán-Carrillo J,Dia V P,et al.Pepsin-pancreatin protein hydrolysates from extruded amaranth inhibit markers of atherosclerosis in LPS-induced THP-1macrophages-like human cells by reducing expression of proteins in LOX-1 signaling pathway[J].Proteome Science,2014,12(1):1-13.
[9] Andriamihaja M,Guillot A,Svendsen A,et al.Comparative efficiency of microbial enzyme preparations versus pancreatin for in vitro alimentary protein digestion[J].Amino acids,2013,44(2):563-572.
[10] 田伟,冯玉萍,李明生,等.水解乳蛋白的制备及在细胞培养中的初步应用[J].天然产物研究与开发,2014,26(1):100-104. [11] Xie NN,Liu SS,Wang C,et al.Stability of casein antioxidant peptide fractions during in vitro digestion/Caco-2 cell model:characteristics of the resistant peptides[J].Eur Food Res Technol,2014,239:577-586.
[12] Jrad Z,EI Hatmi H,Adt I,et al.Effect of digestive enzymes on antimicrobial,radical scavenging and angiotensin I-converting enzyme inhibitory activities of camel colostrum and milk proteins Dairy[J].Sci&Technol,2014,94:205-224.
[13] Balasubramaniam V,Mustar S,Khalid N M,et al.Inhibitory activities of three Malaysian edible seaweeds on lipase andα-amylase[J].Journal of Applied Phycology,2013,25(5):1405-1412.
[14] Gestin M,Le Hurou-Luron I,Peiniau J,et al.Method of measurement of pancreatic elastase II activity and postnatal development of proteases in human duodenal juice and bovine and porcine pancreatic tissue[J].Digestive Diseases and Sciences,1997,42(6):1302-1311.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
