Study on screening a high-yield Bacillomycin strain by UV mutagenesis and its application in preventing rice mildew caused by Aspergillus Flavus
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摘要: 本研究以Bacillus subtilis fmb J为出发菌株,通过紫外诱变选育高产抗菌肽Bacillomycin的菌株,并将Bacillomycin应用于防治大米黄曲霉霉变。结果表明,当紫外照射时间为60 s和80 s时,菌株致死率分别为85.2%和86.8%,正突变率相对较高分别为40.33%和44.87%。进一步采用紫外照射时间60 s和80 s进行诱变,经过筛选得到一株遗传性状稳定的高产Bacillomycin的菌株UV60-4,产量高达306.31 mg/L,是出发菌株产量的1.32倍。在温度为37℃、加水量为6 m L、黄曲霉（3.3×103cfu/m L）接种量为500μL的条件下,Bacillomycin添加量达到0.15 mg/g时,可以有效抑制大米黄曲霉霉变。Abstract: Using Bacillus subtilis fmb J as the starting strain,a mutant strain with high- yield Bacillomycin was acquired by UV mutagenesis,and Bacillomycin was applied to prevent rice from Aspergillus Flavus mildew. The results showed when the fmb J strain was irradiated by UV for 60 s and 80 s,the fatality rate separately reached85.2% and 86.8%,and the higher positive mutation rate was obtained which was 40.33% and 44.87% respectively.Under the UV irradiation time of 60 s and 80 s,a stable and high- yield strain UV60- 4 was selected out with the maximum production of Bacillomycin up to 306.31 mg / L,which was 1.32 times of the starting strain. When the temperature was 37 ℃,the addition of water was 6 m L,and the inoculation of Aspergillus flavus( 3.3 × 103 cfu / m L)was 500 μL,adding 0.15 mg / g Bacillomycin into rice can effectively inhibit mildew caused by Aspergillus flavus.
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Keywords:
- UV mutagenesis /
- fatality rate /
- positive mutation rate /
- prevent /
- mildew
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[1] WANG Jing,ZHU Jianhua,UN Wei,et al.Screening for the Strain Highly Producing Antagonistic Substance from Bacillus subtilis B47 by UV Mutagenesis[J].Agncultura I Science&Techno Iogy,2008,9(4):68-72.
[2] 许向华,史军花,郑鸿雁,等.紫外诱变枯草芽孢杆菌选育葡萄糖-1-磷酸高产菌株[J].中国乳品工业,2011,39(9):21-23. [3] Banat I M,Makkar R S,Cameotra S S.Potential commercial applications of microbial surfactants[J].Microbiology and Biotechnology,2000,53:495-508.
[4] Ibrahim M,Banat,Andrea Franzett,et al.Microbial biosurfactants production,applications and future potential[J].Appl Microbiol Biotechnol,2010,87:427-444.
[5] 王东,王雅玲,孙立军.纳豆菌抗菌脂肽固态发酵工艺优化及其在对虾保鲜上的应用[D].湛江:广东海洋大学,2012:29-33. [6] Hiradate S,S Yoshida,H Sugie,et al.Mulberry anthracnose antagonists(iturins)produced by Bacillus amyloliquefaciens RC-2[J].Phytichemistry,2002,61:693-698.
[7] 罗楚平,刘邮洲,吴荷芳,等.脂肽类化合物bacillomycin L抗真菌活性及其对水稻病害的防治[J].中国生物防治学报,2011,21(1):76-81. [8] Tanaka K,Ishihara A,Nakajima H.Isolation of anteiso-C-17,iso-C-17,iso-C-16,and iso-C-15 Bacillomycin D from Bacillus amyloliquefaciens SD-32 and Their Antifungal Activities against Plant Pathogens[J].Journal of Agricultural and Food,2014,62(7):1469-1476.
[9] Yuan Jun,Li Bing,Zhang Nan.Production of Bacillomycinand Macrolactin-Type Antibiotics by Bacillus amyloliquefaciens NJN-6 for Suppressing Soilborne Plant Pathogens[J].Journal of Agricultural and Food,2012,60(12):2976-2981.
[10] Bluma RV,Etcheverry MG.Influence of Bacillus spp.isolated from maize agroecosystemon growth and aflatoxin B1 production by Aspergillus section Flavi[J].Pest Management Science,2006,62:242-251.
[11] Zhang Q,Li L,Zhu M,et al.Primary cutaneous aspergillosis due to Aspergillus flavus:a case report[J].Chinese Medieal Journal,2005,18(3):255-257.
[12] Oakley EJ,Schmitt F,Jost JP,et al.Some Naturally Occurring Substances:Food items and Constituents,Heterocyclic Aromatic Amines and mycotoxins[J].IARC Scientific Publication,1993,56(6):489-521.
[13] Gong Qingwei,Zhang Chong,Lu Fengxia,et al.Identification of bacillomycin D from Bacillus subtilis fmb J and its inhibition effects against Aspergillus flavus[J].Food Control,2014,36(1):8-14.
[14] 李平兰,贺稚非.食品微生物学实验原理与技术[M].北京:中国农业出版社,2005:263-265. [15] 龚庆伟.芽孢杆菌抗菌脂肽的分离纯化及Bacillomycin D抑制黄曲霉作用的研究[D].南京:南京农业大学,2012:98. [16] 周显青,张玉荣.储藏稻谷品质指标的变化及其差异性[J].农业工程学报,2008,24(12):238-242. [17] 杨晓蓉,周建新,姚明兰,等.不同储藏条件下稻谷脂肪酸值变化和霉变相关性研究[J].粮食储藏,2006,35:49-52. [18] 周建新,鞠兴荣,孙肖东,等.不同储藏条件下稻谷霉菌区系演替的研究[J].中国粮油学报,2008,23(5):133-136.
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