Study on the ultrasonic- assisted extraction of total flavonoids from Xanthoceras Sorbifolia hull and their antioxidant activities
-
摘要: 采用超声波辅助提取文冠果壳总黄酮,并对总黄酮进行抗氧化性研究。通过单因素和响应面实验确定了文冠果壳总黄酮的最佳提取工艺条件:原料粒度为250~178μm、液料比43∶1(m L:g)、乙醇体积分数40%、超声时间19 min、超声功率400 W,在此条件下总黄酮的得率为6.502%,与模拟预测值高度吻合。抗氧化性的研究结果表明:文冠果壳总黄酮具有较强的还原能力和清除DPPH自由基的能力,对·OH的清除能力相对较弱。研究结果可为文冠果壳总黄酮的综合开发利用提供理论基础。Abstract: Ultrasonic assisted extraction technology of total flavonoids from Xanthoceras Sorbifolia hull,and the antioxidant activities of total flavonoids were studied.The optimum extraction conditions for the total flavonoids were established by single factor experiment and response surface experiment. The results showed that the optimal extraction conditions of the total flavonoids were the raw material granularity of 250~178 μm,the solvent- to- solid of43∶1( m L∶ g),the ethanol concentration of 40%,the ultrasonic time of 19 min,the ultrasonic power of 400 W. Under the conditions,the extraction yield of total flavonoids was 6.502%. The result was consistent with the simulated predictive value.The results of antioxidant activities showed that the total flavonoids from Xanthoceras Sorbifolia hull had strong activity of DPPH free radicals and reducing power,whereas it had weaker activity on scavenging activity on hydroxyl radical.The results can be used to provide theoretical basis for the development and utilization of the total flavonoids of Xanthoceras Sorbifolia hull.
-
Keywords:
- Xanthoceras Sorbifolia hull /
- total flavonoids /
- ultrasonic /
- extraction technology /
- antioxidant
-
[1] 张洪梅,周泉城.文冠果壳开发利用研究进展[J].中国粮油学报,2012,27(11):118-121. [2] Adeneye A.Hypoglycemic and hypolipidemic effects of methanol seed extract of Citrus paradise Macfad(Rutaceae)in alloxan-induced diabetic Wistar rats[J].Nigerian quarterly journal of hospital medicine,2008,18(4):211-215.
[3] 林敏,吴冬青,任雪峰,等.基于响应面分析法优化的蕨茎叶黄酮提取条件[J].食品工业科技,2013,34(23):211-215. [4] 周达,罗成,鲁晓翔.玫瑰花总黄酮微波辅助提取及其抗氧化研究[J].食品工业科技,2010,31(4):269-272,276. [5] 李加兴,孙金玉,陈双平,等.超临界CO2萃取杜仲叶黄酮类化合物工艺优化[J].食品工业科技,2013,34(7):252-255,259. [6] 张圣燕,张成.纤维素酶提取冬枣叶中总黄酮工艺的研究[J].食品工业科技,2013,34(1):188-190. [7] 杜蕾,李新华,张振.超声波辅助提取黑花生衣黄酮工艺优化[J].食品工业科技,2013,34(17):259-263. [8] 王鹏,田春莲,田启建.超声波预处理浸提飞龙掌血多糖的工艺优化[J].林产化学与工业,2013,33(4):108-112. [9] 陈小强,张莹,于雪莹,等.超声波辅助法提取黄檗叶片中总黄酮[J].东北林业大学学报,2007,35(9):70-72. [10] 乔孟,屈晓清,丁之恩.响应面法优化超声波辅助提取湖北海棠叶中总黄酮工艺[J].食品科学,2013,34(2):143-147. [11] 谷肄静,王立娟.蒲公英总黄酮的提取及其抑菌性能[J].东北林业大学学报,2007,35(8):43-45. [12] 孟娜,魏胜华,陶玉贵,等.超声波-酶法联合提取绞股蓝总黄酮及其抗氧化活性的研究[J].食品工业科技,2014,35(3):138-141. [13] 祝子坪,李娜.桑黄菌多糖体外抗氧化作用[J].食品科学,2011,32(19):92-95. [14] 郑燕菲,刘雄民,郭占京,等.巨尾桉叶总黄酮提取条件及其抗氧化抑菌研究[J].食品工业科技,2014,35(13):120-124. [15] 董发明,白喜婷.响应面法优化超声提取杜仲雄花中黄酮类化合物的工艺参数[J].食品科学,2008,29(8):227-231.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
