Characterization and biofilm forming-ability of Vibrio alginolyticus
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摘要: 采用改良的微孔板法对114株溶藻弧菌的生物被膜形成能力强弱进行测定,挑选其中1株最强被膜形成能力的菌株进行不同环境因素下的特性分析,并用荧光显微镜观察被膜形成情况。结果表明:测定菌株中有112株具有被膜形成能力（98.25%）,其中具有强或中等强度被膜形成能力的有65株（57.02%）。28℃条件下,溶藻弧菌在含3%Na Cl、p H为6.008.00的TSB中培养16 h时生物被膜的形成量最大;而添加一定浓度的Mn2+、Cu2+、Ca2+、Mg2+后则不同程度地抑制被膜的形成,并且抑制效果依次降低;菌株在接触亲水性表面（不锈钢和玻璃）时被膜形成量显著高于疏水性表面（聚苯乙烯）（p<0.05）,且在不锈钢表面形成量最大;荧光显微镜下的观察结果与微孔板法一致。不同溶藻弧菌菌株生物被膜的形成能力具有很大差异且受不同环境因素的影响。研究结果可以为有效控制溶藻弧菌被膜形成提供理论支持。Abstract: Modified microtiter-plate test was used to investigate the biofilm formation of 114 Vibrio alginolyticus strains. The strongest biofilm formation strain was selected for biofilm characteristics study under various environmental factors. Further,the biofilm formation was observed by using fluorescence microscopy. The results showed that among all the tested strains,112(98.25%) had biofilm formation ability,and 65(57.02%)were at the strong or moderate level. V. alginolyticus formed strong biofilm in p H6.008.00 TSB with 3% Na Cl at 28 ℃ for 16 h. The ability of biofilm formation was inhibited by adding a certain concentration of Cu2+,Mn2+,Ca2+,Mg2+. It's also found that the bacteria adhered to hydrophilic surfaces(stainless steel and glass) better than to hydrophobic ones(polystyrene),and adhered best to stainless steel sheet. The fluorescence microscope observation was consistent with the modified microtiter-plate results. It concluded that V. Alginolyticus biofilm forming-ability was affected by various environmental factors with diversity among different strains. The results provided a theoretical support for the effective control of V. Alginolyticus biofilm formation.
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Keywords:
- Vibrio alginolyticus /
- biofilm /
- environmental factors /
- characteristic
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