Separation, purification and antioxidant activity of polyphenols from Sanguisorba officinalis
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摘要: 通过比较7种大孔树脂对地榆多酚的吸附率和解吸率的影响,筛选出XAD-8树脂适宜分离地榆多酚。地榆多酚分离纯化的条件为:上样浓度2.5 mg/m L,p H5.0,平衡吸附时间3 h,洗脱液乙醇体积分数60%,上样流速1.0 m L/min,洗脱流速1.5 m L/min,纯化后地榆多酚纯度由20.79%提高到62.97%。地榆多酚具有较强的抗氧化能力,清除羟自由基和还原能力均高于VC,地榆多酚对羟自由基和DPPH自由基的半抑制质量浓度（IC50）分别为0.179 mg/m L和0.691 mg/m L。Abstract: Through comparing the adsorption and desorption rates of 7 kinds of macroporous resin to polyphenols, the XAD-8 resin was selected to purify polyphenols of Sanguisorba officinalis. The optimal purification conditions were the sample concentration of 2.5 mg / m L, p H5.0, the adsorption equilibrium of 3 h, ethanol elution volume fraction of 60%, the sample flow rate of 1.0 m L / min, elution flow rate 1.5 m L / min. After purification of Sanguisorba officinalis polyphenols, the polyphenol purity were increased from 20.79% to 62.97%. Polyphenols had strong antioxidant capacity, the scavenging hydroxyl free radical activities and reducing activities of it were superior to VC, the IC50 values of hydroxyl radical and DPPH radical were respectively 0.179 mg / m L and 0.691 mg / m L.
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Keywords:
- Sanguisorba officinalis /
- polyphenols /
- purification /
- antioxidant
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