Optimization of medium for bacterial cellulose fermentation with pomace by response surface methodology and property studies of the product
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摘要: 为提高木醋杆菌（Acetobacter xylinum）发酵苹果渣水解液生产细菌纤维素（Bacterial cellulose,BC）的产量,采用响应面法对发酵培养基进行优化,同时利用傅里叶红外光谱（FT-IR）和X-射线衍射（XRD）对发酵产物BC的性能和结构进行比较。单因素及响应面实验结果确定木醋杆菌（Acetobacter xylinum）发酵苹果渣水解液生产BC的最佳培养基配方为:蔗糖38.44 g、蛋白胨10.91 g、硫酸镁0.85 g、黄嘌呤0.87 g、乙醇10 m L、苹果渣水解液1000 m L、p H6.0,在此条件下BC的产量为7.19 g/L,较优化前（5.65 g/L）提高了27.3%。苹果渣水解液发酵产物BC结构性能与基本培养基发酵产物BC基本一致。说明苹果渣能够替代部分发酵原料发酵生产BC,且不影响BC性能。Abstract: In order to increase the bacterial cellulose( BC) productivity of Acetobacter xylinum using pomace hydrolysate,response surface methodology was used to optimize the fermentation medium. Meanwhile,the properties of BC were compared through fourier transform infrared( FT- IR) and X- ray diffraction( XRD).According to the results of single factor experiments and response surface experiments,the optimal fermentation medium were determined as follows: saccharose 38.44 g,peptone 10.91 g,magnesium sulfate 0.85 g,xanthine 0.87 g,ethanol 10 m L,pomace hydrolysate 1000 m L and p H6.0,under these conditions,the BC productivity was 7.19 g / L,increased by 27.3% in comparision to before( 5.65 g / L). The properties and structures of BC produced with pomace hydrolysate were confirmed to be basically same with BC produced with basic medium,which indicated that the pomace hydrolysate could be used as part of raw materials to ferment BC and would not affect the properties of BC.
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Keywords:
- pomace /
- bacterial cellulose /
- medium optimization /
- response surface methodology
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