Antioxidative and ACE- inhibitory activity of enzymatic hydrolysates of jellyfish Rhopilema esculentum Kishinouye gonad
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摘要: 为实现海蜇加工副产物生殖腺的高值化利用,以海蜇性腺脱脂粉为原料,选用中性蛋白酶、木瓜蛋白酶、胰蛋白酶和碱性蛋白酶分别对海蜇生殖腺进行水解,以酶解产物（JGHP）的水解度、DPPH清除活性和ACE抑制活性为指标,选择制备海蜇生殖腺活性肽的工具酶。经超滤膜将酶解产物分成JGPH-P1（大于3000 u）、P2（10003000 u）、P3（小于1000 u）三个部分,分别测定三部分的DPPH清除活性和ACE抑制活性。结果表明,中性蛋白酶水解所得产物的水解度、DPPH清除率和ACE抑制活性均优于其他三种蛋白酶;JGHP超滤后,其中组分JGHP-P3的活性较高。利用电子自旋共振（ESR）技术检测JGHP-P3对DPPH·、·OH、O-2·的清除活性。其ACE抑制活性IC50为1.06 mg/m L,DPPH·、·OH、O-2·的清除活性IC50分别为0.38、0.63、0.59 mg/m L。由此可见,经中性蛋白酶水解得到的JGPH中小于1000 u的组分具有较高的抗氧化和ACE抑制活性。Abstract: To obtain antioxidant peptides and antihypertensive peptides from Jellyfish Rhopilema esculentum Kishinouye gonad protein hydrolysate( JGPH),Trypsin,Papain,Alcalase and Neutrase were employed for enzymatic hydrolysis. Then degree of hydrolysis,antioxidant activities and antihypertensive activities of their hydrolysates were investigated using both 1,1- diphenyl-2- picrylhydrazyl( DPPH) radical scavenging assay and angiotensin- I- converting enzyme( ACE) inhibition assay. JGPH was ultrafiltrated using ultrafiltration membranes( molecular weight cut-offs of 1000 and 3000 u) to obtain three components JGPH-P1-3 which molecular weight distributions were > 3000 u,1000 ~3000 u and < 1000 u,then antioxidant activities and antihypertensive activities of three components were investigated. The results revealed that degree of hydrolysis,DPPH radical scavenging activity and ACE- inhibitory activity of JGPH prepared by Neutrase were the highest,and Neutrase was selected as the tool enzyme.JGPH- P3 had the highest DPPH radical scavenging activity and ACE- inhibitory activity( IC50=1.06 mg / m L). And its DPPH radical scavenging activity( IC50= 0.38 mg / m L),hydroxyl radical scavenging activity( IC50= 0.63 mg / m L) and superoxide anion radical scavenging activity( IC50= 0.59 mg / m L) were investigated by using electron spin resonance measurement( ESR).Thus,molecular weight less than 1000 u of of JGPH prepared by Neutrase exhibited high antioxidant activities and antihypertensive activities.
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Keywords:
- jellyfish /
- gonad /
- peptides /
- antioxidant /
- ACE
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