Optimization of enzymatic hydrolysis conditions for myofibrillar protein of chicken breast by response surface methodology
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摘要: 以鸡胸肉为原料,提取肌原纤维蛋白,测定其蛋白质浓度及氨基酸组成;以水解度为测定指标,利用响应面法优化该肌原纤维蛋白酶解工艺条件,并考察其产物肽分子量分布。结果表明:提取的肌原纤维蛋白浓度为59.27%;该蛋白氨基酸种类齐全,组成比例均衡,必需氨基酸含量高达40.90%;碱性蛋白酶是肌原纤维蛋白酶解的最佳用酶;在单因素实验基础上,经Box-Behnken实验优化得到碱性蛋白酶水解肌原纤维蛋白最佳工艺条件为:加酶量3500 U/g、p H7.8、温度44℃,此条件下经6 h酶解,水解度达33.17%;肽分子量分布分析知,最佳水解条件下酶解液中<1000 u的小肽含量高达61.5%,说明碱性蛋白酶能较高程度的水解肌原纤维蛋白。Abstract: Based on the degree of hydrolysis,enzymatic hydrolysis conditions of the myofibrillar protein extracted from chicken breast was optimized by response surface methodology in this article,the protein content and amino acid composition of the myofibrillar protein and the molecular weight distribution of the hydrolysates were also measured.Results showed that protein content of the extracted myofibrillar was 59.27%,the types and composition ratio of amino acids in this protein was every inch and balanced,the content of essential amino acid reached40.90%. The best enzyme for hydrolyzing myofibrillar protein was alcalase. On the foundation of single factors experiment,the optimal hydrolysis conditions for myofibrillar protein hydrolyzed by alcalase was obtained as:enzyme concentration 3500 U / g,p H7.8 and temperature 44 ℃ through the Box- Behnken methodology. After hydrolysis for 6 h under the above conditions,the DH reached 33.17%. The content of peptide < 1000 u reached61.5% by peptide molecular weight distribution,illustrated that myofibrillar proteins hydrolyzed in a high degree by alcalase.
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