Screening and identification of antioxidant producing marine- source actinomycetes
-
摘要: 采用传统的分离培养技术对4种海洋动物肠道中的放线菌进行分离,利用紫外-可见分光光度法对分离菌株的代谢产物进行抗氧化活性初筛测定,再采用电子自旋共振仪（ESR）进行4种自由基清除率复筛测定,并对抗氧化活性强的菌株进行16S r DNA水平鉴定与系统发育树分析。结果表明共分离到37株放线菌,初筛测定获得7株菌的代谢产物对DPPH自由基清除率超过50%,其中菌株CH16与CH38的代谢产物通过ESR测定发现对DPPH自由基、羟基自由基、超氧阴离子自由基及烷基自由基的消除率均超过50%,并分别鉴定为Amycolatopsis rifamycinica与Amycolatopsis sp.。该研究为从海洋环境获得产生抗氧化活性物质菌株资源与开发提供依据。Abstract: Actinomycetes were isolated from the gastrointestinal tract of four kinds of marine animals by classical culturing technique.Actinomycetes was primarily screened with the method of UV- visible spectrometry to detect the antioxidant activity,then four radical scavenging( DPPH radical,hydroxyl radical,superoxide radical and alkyl radical) were further determined by JES- FA electron spin resonance( ESR) spectrometer.Finally,strains with higher antioxidant activity were identified by 16 S r DNA. Results showed that 37 actinomycetes were acquired,and DPPH radical scavenging rates of seven strains were found over 50% by primarily screening method,and DPPH radical scavenging,hydroxyl radical scavenging,superoxide radical scavenging,alkyl radical scavenging of CH16 and CH38 were higher than 50% by ESR method. Also the strain CH16 and CH38 were identified as Amycolatopsis rifamycinica and Amycolatopsis sp.,respectively. This research can provide reference for collecting having antioxidant activity marine actinomycetes resource and further development.
-
Keywords:
- antioxidant activity /
- metabolic product /
- marine actinomycete /
- screening /
- identification
-
[1] 陈艳丽,任盛,魏国琴,等.Rhizobium sp.N613胞外多糖的抗氧化活性[J].微生物学通报,2010,37(2):235-238. [2] 姜俊杰,邢莹莹,陆园园,等.北极放线菌T-2-3胞外多糖体外抗氧化作用研究[M].南京:药物生物技术,2012,19(4):313-316. [3] Kim M C,Lee J,Kim D H,et al.Isolation and identification of antioxidant producing marine-source actinomycetes and optimal medium conditions[J].Food science and Biotechnology.2014,3(5):1629-1635.
[4] Sehinella G R,Tournier H A,Prieto J M,et al.Antioxidant activity of anti-inflammatory plant extracts[J].Life sciences,2002,70:1023-1033.
[5] 卢虹玉,刘义,吉宏武,等.全缘马尾藻褐藻多酚的抗氧化和抗肿瘤细胞增殖作用研究[J].现代食品科技,2013,29(4):702-705. [6] Karthik L,Kumar G,Bhaskara Rao KV.Antioxidant activity of newly discovered lineage of marine actinobacterias[J].Asian Pacific Journal of Tropical Medicine,2013,6:325-332.
[7] Arumugam M,Mitra A,Jaisankar P,et al.Isolation of an unusual metabolite 2-allyloxyphenol from a marine actinobacterium,its biological activities and applications[J].Applied Microbiology and Biotechnology,2010,86:109-117.
[8] 廖振林,刘箐,陈建宏,等.广西北海红树林土壤放线菌的分离与鉴定[J].安徽农业科学,2010,38(23):12693-12694,12702. [9] 胡喜兰,韩照样,陶莹,等.DPPH法测定17种植物的抗氧化活性[J].食品科技,2006:264-268. [10] Kim S Y,Kim J H,Kim S K.Antioxidant activities of selected oriental herb extracts[J].Journal of the American Oil Chemists Society,1994,71(6):633-640.
[11] Qian Z J,Ryu B,Kim M M,et al.Free radical and reactive oxygen species scavenging activities of the extracts from seahorse,Hippocampus kuda Bleeler[J].Biotechnology and Bioprocess Engineering,2008,13:705-715.
[12] Qian Z J,Jung W K,Byun H G,et al.Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster,Crassostrea gigasagainst free radical induced DNA damage[J].Bioresource Technology,2008,99:3365-3371.
[13] Lu Q F,Hu H Q,Mo J J,et al.Enhanced amplification of bacterial and fungal DNA using a new type of DNA polymerase[J].Australasian Plant Pathol,2012,41:661-663.
[14] Carocho M,Ferreira I C.A review on antioxidants,prooxidants and related controversy:natural and synthetic compounds,screening andnalysis methodologies and future perspectives[J].Food Chem.Toxicol.2013,51:15-25.
[15] Park E J,Pezzuto J M.Antioxidant marine products in cancer chemoprevention[J].Antioxid Redox Signal,2013,19:115-138.
[16] Li Y,Xu Y,Liu L,et al.Five new amicoumacins isolated from a marine-derived bacterium Bacillus subtilis[J].Marine Drugs.2012,10:319-328.
[17] Rastogi R P,Incharoensakdi A.UV radiation-induced biosynthesis,stability and antioxidant activity of mycosporine-like amino acids(MAAs)in a unicellular cyanobacterium Gloeocapsa sp.CU2556[J].Journal of Photochemistry and Photobiology B:Biology,2014,130(5):287-316.
[18] Ikeda H,Ishikawa J,Hanamato A,et al.Complete genome sequence and comparative analysis of the industrial microorganism Stretomyces avermitilis[J].Nature Biotechnology,2003,21:526-531.
[19] Shin-ya K.Screening of antioxidative agents of microbial origin and their application[J].Actinomycetologica,1994,8(1):S8.
[20] Kumar Saurav,Krishnan Kannabiran.Cytotoxicity and antioxidant activity of 5-(2,4-dimethylbenzyl)pyrrolidin-2-one extracted from marine Streptomyces VITSVK5 spp[J].Saudi Journal of Biological Sciences,2012,19:81-86.
[21] Thenmozhi M,Kannabiran K.Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp VITSTK7[J].Oxidants and Antioxidants in Medical Science,2012,1(1):51-57.
[22] 孙海红.三株海洋微生物中胞外多糖的分离、结构和抗氧化活性研究[D].青岛:中国海洋大学,2009.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
