Study on extraction,purification and antitumor activity of polysaccharide from bee pollen of Dendranthema indicum
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摘要: 本文对蜂花粉多糖进行了分离纯化和组成分析,并初步分析了各多糖级分对肿瘤细胞增殖的抑制作用。选取菊花蜂花粉为原料,通过水提、醇沉得到总多糖WDPP,再通过离子交换层析得到一个中性糖（WDPP-N）和两个酸性糖级分（WDPP-1,WDPP-2）。通过高效液相色谱法测定各级分的单糖组成,通过对肿瘤细胞增殖作用的影响探讨各级分的抗肿瘤活性。结果表明,WDPP-N主要由半乳糖、阿拉伯糖和葡萄糖组成,比例为30.3∶31.6∶32.7;WDPP-1和WDPP-2都主要由半乳糖醛酸、半乳糖和阿拉伯糖组成,还含有少量的鼠李糖、葡萄糖等。WDPP-N和WDPP-2浓度为5 mg/m L时对肿瘤细胞HCT116增殖的抑制率分别为38%和32%,对HT-29增殖的抑制率为42%和47%,WDPP-1对肿瘤细胞增殖作用的影响不显著（p>0.05）,而WDPP在浓度为5 mg/m L时对HCT116和HT-29肿瘤细胞增殖的抑制率分别为61%和81%。因此,菊花蜂花粉总多糖WDPP抑制肿瘤细胞增殖的活性优于中性糖和酸性糖。Abstract: The purification procedures,physiochemical features and antitumor activity of polysaccharide fractions from bee pollen of Dendranthema indicum were studied in this paper.Polysaccharide fractions( WDPP) was dealed with water-extraction,ethnol-precipetation and ion- exchange chromatography,then one netral( WDPP- N) and two acidic fractions( WDPP-1,WDPP-2) were obtained.The monosaccharide composition was tested through HPLC,the antitumor activity of all fractions were investigated by MTT method.WDPP-N was composed of galactose,arabinose,and glucose with a ratio of 30.3 ∶ 31.6 ∶ 32.7; WDPP- 1 and WDPP- 2 were mainly composed by galacturonic acid,galactose and arabinose,rhamose and glucose as small proportion.Total polysaccharide had obvious inhibition effect on HT-29 and HCT116 tumor cells in high concentration compared with neutral and acidic fractions.
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