Structural characterization of a polysaccharide from cultured Cordyceps militaris with antioxidant activity
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摘要: 采用分子筛层析法从人工培养蛹虫草子实体中分离纯化得到一种多糖W-CBP80Ⅰ,进而分别采用凝胶渗透色谱（GPC）、气-质联用（GC-MS）、红外光谱（IR）、核磁共振(1H NMR和13C NMR)研究其理化性质和结构特征。结果表明:W-CBP80Ⅰ含有α-糖苷键,并主要由甘露糖、半乳糖和葡萄糖组成。它主要成分为低分子量多糖,分子量为8.93×103。此外,W-CBP80Ⅰ具有体外清除DPPH,羟基和超氧阴离子自由基活性。Abstract: A polysaccharide W-CBP80 Ⅰ was isolated from the fruiting bodies of cultured Cordyceps militaris and characterized using GPC,GC-MS,IR,~1H NMR and 13C NMR. The results indicated that it contained α-type glycosidic linkages and was mainly composed of α-mannose,α-galactose and α-glucose. Its main component was low molecular weight polysaccharide with Mwof 8.93×10~3. In addition,W-CBP80Ⅰ exhibited scavenging capacities against DPPH,hydroxyl and superoxide radicals in the antioxidant assay in vitro.
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Keywords:
- Cordyceps militaris /
- polysaccharide /
- structure characterization /
- antioxidation
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[1] Domann F E.Aberrant Free Radical Biology is a Unifying Theme in the Etiology and Pathogenesis of Major Human Diseases[J].International Journal of Molecular Sciences,2013,14(4):8491-8495.
[2] Gutowski M,Kowalczyk S.A study of free radical chemistry:their role and pathophysiological significance[J].Acta Biochim Pol,2013,60(1):1-16.
[3] Stachowiak B,Regula J.Health-promoting potential of edible macromycetes under special consideration of polysaccharides:a review[J].Eur Food Res Technol,2012,234(3):369-380.
[4] Hu D J,Cheong K L,Zhao J,et al.Chromatography in characterization of polysaccharides from medicinal plants and fungi[J].Journal of Separation Science,2013,36(1SI):1-19.
[5] Wu D,Xie J,Wang L,et al.Characterization of bioactive polysaccharides from Cordyceps militaris produced in China using saccharide mapping[J].Journal of Functional Foods,2014,9:315-323.
[6] Chen R,Jin C,Li H,et al.Ultrahigh pressure extraction of polysaccharides from Cordyceps militaris and evaluation of antioxidant activity[J].Sep Purif Technol,2014,134:90-99.
[7] Jing Y S,Cui X L,Chen Z Y,et al.Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris[J].Carbohyd Polym,2014,102:288-296.
[8] Zhu L,Tang Q,Zhou S,et al.Isolation and Purification of a Polysaccharide from the Caterpillar Medicinal Mushroom Cordyceps militaris(Ascomycetes)Fruit Bodies and Its Immunomodulation of RAW 264.7 Macrophages[J].International Journal of Medicinal Mushrooms,2014,16(3):247-257.
[9] Li S P,Zhang G H,Zeng Q,et al.Hypoglycemic activity of polysaccharide,with antioxidation,isolated from cultured Cordyceps mycelia[J].Phytomedicine,2006,13(6):428-433.
[10] Zeng Y,Han Z,Qiu P,et al.Salinity-induced anti-angiogenesis activities and structural changes of the polysaccharides from cultured cordyceps militaris.[J].PLOS ONE,2014,9(9):e103880.
[11] Yu R,Song L,Zhao Y,et al.Isolation and biological properties of polysaccharide CPS-1 from cultured Cordyceps militaris[J].Fitoterapia,2004,75(5):465-472.
[12] 张惟杰.糖复合物生化研究技术[M].第二版.杭州:浙江大学出版社,1999. [13] Chen X L,Wu G H,Huang Z L.Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris[J].Int J Biol Macromol,2013,58:18-22.
[14] 黄晓兰,吴惠勤,黄芳,等.破壁与不破壁灵芝孢子粉多糖的分析[J].中草药,2006,37(6):813-816. [15] Ding X,Hou Y,Hou W.Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst[J].Int J Biol Macromol,2012,50(3):613-618.
[16] Yao L,Zhao Q,Xiao J,et al.Composition and antioxidant activity of the polysaccharides from cultivated Saussurea involucrata[J].Int J Biol Macromol,2012,50(3):849-853.
[17] Bock K,Pedersen C,Pedersen H.Carbon-13 Nuclear Magnetic Resonance Data for Oligosaccharides[M].Academic Press,1984.
[18] Niu Y,Yan W,Lv J,et al.Characterization of a Novel Polysaccharide from Tetraploid Gynostemma pentaphyllum Makino[J].J Agr Food Chem,2013,61(20):4882-4889.
[19] Yu R M,Yang W,Song L Y,et al.Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps militaris[J].Carbohyd Polym,2007,70(4):430-436.
[20] Wu F Y,Yan H,Ma X N,et al.Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media[J].World J Microb Biot,2012,28(5):2029-2038.
[21] Cheng H N,Neiss T G.Solution NMR Spectroscopy of Food Polysaccharides[J].Polymer Reviews,2012,52(2):81-114.
[22] Zhou C,Yu X,Zhang Y,et al.Ultrasonic degradation,purification and analysis of structure and antioxidant activity of polysaccharide from Porphyra yezoensis Udea[J].Carbohyd Polym,2012,87(3):2046-2051.
[23] Jing Y,Cui X,Chen Z,et al.Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris[J].Carbohyd Polym,2014,102:288-296.
[24] Liu F,Ooi V E C,Chang S T.Free radical scavenging activities of mushroom polysaccharide extracts[J].Life Sci,1997,60(10):763-771.
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