Protective effect of Lingonberry extraction on chronic stressed mice
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摘要: 研究红豆越橘提取物对小鼠慢性应激损伤的防护作用。将60只雄性昆明小鼠随机分成6组:正常对照组、模型组、阳性对照组（氟西汀4.4 mg/kg·d）、实验组（红豆越橘低、中、高剂量分别为50、100和200 mg/kg·d）。采用慢性应激模型,从行为学和生理学角度研究红豆越橘的抗应激作用。结果表明:与对照组相比,模型组小鼠悬尾不动时间明显延长（p<0.01）;脑组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、5-羟基色氨（5-HT）含量降低（p<0.05）,丙二醛（MDA）含量升高（p<0.05）;血清去甲基肾上腺素NE和5-HT含量降低（p<0.05）,糖皮质激素（GC）和乙酰胆碱酯酶（AChE）含量升高（分别为p<0.01,p<0.05）。与模型组相比,高剂量组小鼠悬尾不动时间明显缩短（p<0.05）;低剂量组小鼠脑组织MDA含量降低（p<0.05）,高剂量组小鼠脑组织SOD含量、高、低剂量组脑组织5-HT含量以及低剂量组脑组织GSH-Px含量均升高（p<0.05）;高剂量组小鼠的血清GC、ACh E含量降低（p<0.01,p<0.05）,中剂量组血清5-HT含量升高（p<0.05）。因此红豆越橘可一定程度地保护机体,减少慢性应激对小鼠造成的不良影响。Abstract: In order to study the protective effect of Lingonberry extraction on chronic stressed mice,total 60 male Kunming mice were randomly divided into 6 groups :normal control group,stressed model group,positive group( Fluoxetine 4. 4 mg / kg·d), experience group( low, medium, high dose were 50, 100, 200 mg / kg·d respectively). Use the chronic stress animal model to research anti stress effect of Lingonberry in behavior and physiology. Compared with control group,model mice immobility duration significantly prolonged in tail suspended test(p<0.05),cerebral SOD,GSH-Px and 5-HT decreased(p<0.05),MDA increased(p<0.05),serum NE and 5-HT decreased(p <0.05),GC and ACh E increased(p <0.01,p <0.05 respectively). Compared with model group,the mice of high dose group immobility duration significantly shortened in tail suspended test,low dose group mice cerebral MDA reduced(p<0.05),high dose mice cerebral SOD,high and low dose group mice cerebral 5-HT,and low dose group cerebral tissue GSH-Px content increased(p<0.05). High dose group serum GC and ACh E decreased(p <0.01,p <0.05),middle dose group serum 5-HT increased(p <0.05). In conclusion,Lingonberry can greatly protect the body,reduce the adverse effects of chronic stress on mice.
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Keywords:
- Lingonberry /
- chronic stress /
- protective effect /
- mice
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