Extraction of polysaccharides from Phellinus igniarius mycelium and research of its antioxidation activity in vitro
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摘要: 研究了桑黄(Phellinus igniarius)菌丝体多糖热水提取的最佳工艺条件及其体外抗氧化活性。通过单因素实验和正交实验对提取工艺进行优化,考察提取时间、温度和料液比及提取次数对桑黄菌丝体多糖得率的影响。最佳提取工艺条件为温度100℃,时间2 h,料液比1∶15 g/m L,提取2次。在该条件下,桑黄菌丝体多糖得率为6.64%。该粗多糖含量为69.29%,蛋白含量为3.20%。浓度为0.2 mg/m L的桑黄菌丝体多糖对超氧负离子清除率为49.4%,对羟基自由基的清除率为28.7%,还原力为0.072,低于V_C相对应的抗氧化能力。Abstract: In order to obtain the optimal technological condition of polysaccharides extraction from Phellinus igniarius mycelium,and study its antioxidation activity in vitro. Then,the effects of the extraction time,extraction temperature,solid- liquid ratio and the extraction times of the polysaccharides by single factor experiments and orthogonal experiments in distilled water on the yield of this polysaccharide were investigated.At the same time,its scavenging abilities on superoxide and hydroxyl radicals and reducing power were observed. The orthogonal experimental results showed that the optimal conditions were extraction temperature 100 ℃,solid- liquid ratio1∶15 g / m L,extraction time 2 h,2 times.Under this condition,the average rate of extraction of polysaccharides was6.64%.The polysaccharide content was 69.29%,and the protein content was 3.20% of this crude polysaccharide.When the concentration of polysaccharides from P. igniarius mycelium was 0.2 mg / m L,the scavenging rate on superoxide anion was 49.4%,scavenging rate on hydroxyl radical was 28.7%,and reducing power was 0.072.The results were all less than that of VC.
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