Extraction and purification of zearalenone in Fusarium graminearum
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摘要: 本研究旨在通过不同萃取剂和层析柱的使用,从禾谷镰刀菌发酵液中提取、纯化玉米赤霉烯酮（zearalenone,ZEN）毒素,以期获得较高纯度的毒素。采用超高效液相色谱串联质谱（ultra-performance liquid chromatography-mass spectrometry,UPLC-MS）分析方法对其效果进行检测。研究结果表明:在单一的萃取剂中,苯的萃取效果最佳,其得率可达到73.20%;采用Daisogel反相层析柱进行纯化,得率可达到69.30%,且样品中的杂峰数目明显减少,由此证明该方法对毒素纯化效果较好。本研究对发展高纯度ZEN毒素的制备技术具有一定的指导意义和参考价值。Abstract: To obtain highly purified zearalenone,the mycotoxin was extracted and then purified from the fermented liquid of Fusarium graminearum in this study using different extraction solvents and chromatographic columns.The effects of extraction and purification were evaluated by ultra- performance liquid chromatography- mass spectrometry( UPLC- MS).The results demonstrated that benzene was recognized to be the best one of the single extraction agents,ewhose yield could reach up to 73.20%.Daisogel reverse phase chromatography column was much better chosen to purify zearalenone because its yield was up to 69.30% and the numbers of the impurity peaks significantly decreased.The findings of this study can provide the guidance and reference for the development of highly purity of zearalenone.
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Keywords:
- mycotoxin /
- zearalenone /
- Fusarium graminearum /
- extraction /
- purification
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[1] 姜淑贞,杨维仁,杨在宾.玉米赤霉烯酮的代谢、毒性及其预防措施[J].动物营养学报,2011,23(2):196-202. [2] 徐新慧,晏猛,符锋,等.郑州某粮库小麦中玉米赤霉烯酮含量的检测[J].动物医学进展,2011,33(4):59-62. [3] Wang Y,Liu SL,Zheng H,et al.T-2 toxin,zearalenone and fumonisin B1 in feedstuffs from China[J].Food Additives&Contaminants:Part B:Surveillance,2013,6(2):116-122.
[4] 张东升,王虎,蔡建荣,等.玉米副产物中玉米赤霉烯酮的ELISA测定[J].饲料工业,2008,29(1):38-40. [5] 尹青岗,王锋,赵国华,等.谷物中玉米赤霉烯酮去毒方法的研究进展[J].粮食与饲料工业,2008,8(10):42-44. [6] 龙淼,李鹏,朱连勤,等.微生物降解玉米赤霉烯酮毒素及其机制[J].动物医学进展,2011,32(11):116-119. [7] Judith M,Johannes K,Alexander P.In vitro competitive interactions of Fusarium graminearum with Aspergillus ochraceus and Penicillium verrucosum with regard to mycotoxin production[J].Journal of Food,Agriculture and Science,2007,5(3):384-388.
[8] 徐得月,王伟,陈西平,等.禾谷镰刀菌产毒影响因子预测微生物学筛选[J].中国公共卫生,2013,29(1):72-76. [9] 张枫,张蕾,张重阳,等.一株产T-2毒素镰孢菌的分离、鉴定及其产毒条件的研究[J].微生物学通报,2015,42(2):340-348. [10] 甄玉萍,裴世春,高建伟,等.不同培养条件对禾谷镰刀菌产玉米赤霉烯酮的影响[J].食品科学,2015,36(21):168-174. [11] 王滨,张簏,李季伦.玉米赤霉烯酮生物合成条件的研究[J].北京农业大学学报,1985,11(1):5-13. [12] 李汉卿,谢胜学,王雪,等.镰刀菌毒素的研究-(Ι)玉米赤霉烯酮的分离提纯及测定[J].黑龙江大学自然科学学报,1991,8(3):17-23. [13] 甄玉萍,裴世春,王岩,等.玉米样品前处理方法和掩蔽剂对ELISA检测玉米赤霉烯酮的影响[J].食品科学,2015,36(16):255-260. [14] 谢刚,王松雪,崔华,等.超高效液相色谱法快速检测粮食中玉米赤霉烯酮的含量[J].粮油食品科技,2014,22(2):71-75. [15] Choi E H,Kim D M,Chol S W,et al.Analysis of zearalenone andα-zearalenol in animal feed using high-performance liquid chromatography[J].International Journal of Food Science and Technology,2011,46(10):2173-2181.Analytica Chimica Acta,2003,487(2):137-143.
[16] 宋范范,张康逸,张薇薇,等.无水乙醇萃取联合氧化铝柱层析制备高纯磷脂酰胆碱[J].食品科学,2015,36(10):6-10. [17] Urraca J.L.,Marazuela M.D.,Moreno-Bondi M.C..Analysis for zearalenone andα-zearalenol in cereals and swine feed using accelerated solvent extraction and liquid chromatography with fluorescence detection[J].Analytica Chimica Acta,2004,524(1):175-184.
[18] 唐燕文,何卫华,廖霞俐,等.吗啡有效成分多种提取方法的比较研究[J].食品工业科技,2015,36(5):189-193. [19] 王宏杰,徐剑宏,祭芳,等.饲料中玉米赤霉烯酮的提纯与测定[J].江苏农业学报,2011,27(3):663-667. [20] 胡淼,钱国平,苏宝根,等.硅胶柱层析纯化青蒿素[J].华西药学杂志,2005,20(4):283-285. [21] 张志强,苏志国.正相和反相柱层析组合分离纯化紫杉醇[J].生物工程学报,2000,16(1):69-73. [22] 尹珺.脱氧雪腐镰刀菌烯醇(DON)检测方法的研究[D].南京:南京农业大学,2006:38-49. [23] 徐剑宏,史建荣,祭芳,等.DON毒素的制备纯化方法:中国,101914586 A[P].2010-12-15. [24] Kim H J,Kim S H,Lee J K,et al.A novel mycotoxin purification system using magnetic nanoparticles for the recovery of aflatoxin B1 and zearalenone from feed[J].Journal of Veterinary Science,2012,13(4),363-369.
[25] 鲍蕾,吕宁,吴振兴,等.免疫亲和柱同时净化-HPLC法测定植物油中黄曲霉毒素和玉米赤霉烯酮[J].食品工业科技,2013,34(9):306-308. [26] 隋凯,李军,卫锋,等.免疫亲和柱-高效液相色谱法检测玉米中的玉米赤霉烯酮[J].中国卫生检验杂志,2006,16(6):657-690. [27] Filipe O M S,Vidal M M,Duarte A C,et al.Influence of fulvic acids and copper ions on thiram determination in water[J].Journal of Agricultural and Food Chemistry,2008,56(16),7347-7354.
[28] Raquel M,JoséA.G M.Rapid and quantitative extraction method for the determination of chlorophylls and carotenoids in olive oil by high-performance liquid chromatography[J].Analytical and Bioanalytical Chemistry,2006,385(7),1247-1254.
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