Research of technology of extraction oil from rice bran with ultrasonic assisted aqueous enzymatic
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摘要: 为了提高米糠的出油率,本文采用超声波辅助水酶法,对混合液进行破乳,得到最佳工艺条件。通过研究复合酶的种类、比例、添加量以及酶解条件对米糠出油率的影响,在单因素的基础上,进行Box-Benhnken响应面实验。结果表明超声波辅助水酶法提取米糠油的最佳条件为:料液比1∶6,酶解温度设定为51℃,酶解时间5 h,超声波功率230 W,超声时间20 min,酶添加量为1.38%（碱性蛋白酶∶纤维素酶∶果胶酶=1∶2∶1）。此条件下得到米糠的出油率为90.53%。Abstract: To improve the rice bran oil yield,using ultrasonic assisted aqueous enzymatic method and the demulsify the emulsion,the optimum technological conditions were obtained.The effect of the type,proportion,dosage of enzyme and the hydrolysis conditions on the extraction of rice bran oil was investigated.On the basis of single factor,the Box- Benhnken response surface experiment was carried out.The optimum parameters of the extraction process was solid- to- liquid ratio of 1 ∶ 6,hydrolysis temperature of 51 ℃,hydrolysis time of 5 h,the ultrasonic power of 230 W,ultrasonic time of 20 min,hydrolysis dosage of 1.38%( alkaline protease ∶ compound cellulase ∶pectinase = 1∶ 2∶ 1).Under these optimal hydrolysis conditions,the yield efficiency of rice bran oil was up to 90.53%.
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Keywords:
- rice bran oil /
- ultrasound /
- aqueous enzymatic /
- response surface
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