Progress in detection methods for epitopes related to celiac disease in wheat gluten
-
摘要: 乳糜泻关联表位的定量检测可以评价小麦种质资源及加工食品的乳糜泻致敏性,为患者安全消费提供技术保障。定量检测小麦麸质蛋白中致乳糜泻表位的方法包括免疫印迹法、高通量测序法和多反应检测串联质谱检测（LC-MRM/MS）,它们在麸质表位的检测中各具特色。免疫印迹法检测结果稳定直观,但定量精确度不高;高通量测序法准确度相对较高,但应用范围窄,且数据处理困难;多反应检测串联质谱检测灵敏度高、样品用量少、分析速度快,但费用较高。Abstract: Detection of epitopes in gluten related to celiac disease can evaluate the sensitivity of wheat variety resources and the relevant food,and it is a strong technical support for the safety consumption for celiac disease patients.The main methods used in the detection of epitopes related to celiac disease in wheat gluten involve three approaches based on western blotting,next- generation sequencing,LC- MRM / MS,respectively.The advantages and disadvantages of these methods were introduced in detail.Among them,western blotting could present direct results,while the quantitative accuracy was not high.The accuracy of next- generation sequencing is relatively high,while the range of application is narrow with a complicated data processing.LC- MRM / MS has advantages on high sensitivity,low sample consumption and fast analysis speed with higher cost.
-
Keywords:
- celiac disease /
- T cell epitope /
- gluten
-
[1] Mcallister C S,Kagnoff M F.The immunopathogenesis of celiac disease reveals possible therapies beyond the gluten-free diet[J].Semin Immunopathol,2012,34(4):581-600.
[2] 于凤莲,李素云,袁娟丽,等.小麦α-麦醇溶蛋白中致乳糜泻肽段的研究进展[J].食品工业科技,2014,35(2):364-368. [3] Shewry P R,Halford N G.Genetics of wheat gluten proteins[J].Advances in Genetics,Vol 49,2003,49:111-184.
[4] Sollid L M,Qiao S W,Anderson R P,et al.Nomenclature and listing of celiac disease relevant gluten T-cell epitopes restricted by HLA-DQ molecules[J].Immunogenetics,2012,64(6):455-460.
[5] Shan L,Molberg O,Parrot I,et al.Structural basis for gluten intolerance in Celiac sprue[J].Science,2002,297(5590):2275-2279.
[6] Garcia E,Llorente M,Hernando A,et al.Development of a general procedure for complete extraction of gliadins for heat processed and unheated foods[J].European Journal of Gastroenterology&Hepatology,2005,17(5):529-539.
[7] Mendez E,Vela C,Immer U,et al.Report of a collaborative trial to investigate the performance of the R5 enzyme linked immunoassay to determine gliadin in gluten-free food[J].European Journal of Gastroenterology&Hepatology,2005,17(10):1053-1063.
[8] Gessendorfer B,Wieser H,Koehler P.Optimisation of a solvent for the complete extraction of prolamins from heated foods[J].Journal of Cereal Science,2010,52(2):331-332.
[9] Shan L,Qiao S W,Arentz-Hansen H,et al.Identification and analysis of multivalent proteolytically resistant peptides from gluten:Implications for Celiac Sprue[J].Journal of Proteome Research,2005,4(5):1732-1741.
[10] Sapone A,Lammers K M,Casolaro V,et al.Divergence of gut permeability and mucosal immune gene expression in two glutenassociated conditions:celiac disease and gluten sensitivity[J].BMC Med,2011,9.
[11] Molberg,Uhlen A K,Jensen T,et al.Mapping of gluten T-cell epitopes in the bread wheat ancestors:Implications for celiac disease[J].Gastroenterology,2005,128(2):393-401.
[12] Mitea C,Salentijn E M J,Van Veelen P,et al.A Universal Approach to Eliminate Antigenic Properties of Alpha-Gliadin Peptides in Celiac Disease[J].Plos One,2010,5(12).
[13] Constantin C,Touraev A,Heberle-Bors E,et al.Detection of Antigens Reactive to Ig E and Ig A during Wheat Seed Maturation and in Different Wheat Cultivars[J].International Archives ofAllergy and Immunology,2009,149(3):181-187.
[14] Van Den Broeck H,Chen H B,Lacaze X,et al.In search of tetraploid wheat accessions reduced in celiac disease-related gluten epitopes[J].Mol Biosyst,2010,6(11):2206-2213.
[15] 朱晓燕.基于T细胞表位筛选低乳糜泻致敏性小麦品种[D].南昌:南昌大学,2013. [16] 吕崇富.挤压加工对面粉麸质蛋白结构和致敏性的影响[D].南昌:南昌大学,2014. [17] 于凤莲.新疆小麦及主要面制品馕的乳糜泻致敏性评估[D].南昌:南昌大学,2015. [18] Quail M A,Kozarewa I,Smith F,et al.A large genome center’s improvements to the Illumina sequencing system[J].Nature Methods,2008,5(12):1005-1010.
[19] Meyer M,Stenzel U,Hofreiter M.Parallel tagged sequencing on the 454 platform[J].Nature Protocols,2008,3(2):267-278.
[20] Mardis E R.The impact of next-generation sequencing technology on genetics[J].Trends in Genetics,2008,24(3):133-141.
[21] Christiansen A,Kringelum J V,Hansen C S,et al.Highthroughput sequencing enhanced phage display enables the identification of patient-specific epitope motifs in serum[J].Scientific Reports,2015,5.
[22] Salentijn E M J,Esselink D G,Goryunova S V,et al.Quantitative and qualitative differences in celiac disease epitopes among durum wheat varieties identified through deep RNA-amplicon sequencing[J].BMC Genomics,2013,14.
[23] Knapp D J H F,Mcgovern R A,Poon A F Y,et al.“Deep”Sequencing Accuracy and Reproducibility Using Roche/454Technology for Inferring Co-Receptor Usage in HIV-1[J].Plos One,2014,9(6).
[24] Van Herpen T W J M,Goryunova S V,Van Der Schoot J,et al.Alpha-gliadin genes from the A,B,and D genomes of wheat contain different sets of celiac disease epitopes[J].BMC Genomics,2006,7.
[25] Anderson O D,Greene F C.The alpha-gliadin gene family.2.DNA and protein sequence variation,subfamily structure,and origins of pseudogenes[J].Theoretical and Applied Genetics,1997,95(1-2):59-65.
[26] Ozuna C V,Iehisa J C M,Gimenez M J,et al.Diversification of the celiac disease-gliadin complex in wheat:a 33-mer peptide with six overlapping epitopes,evolved following polyploidization[J].Plant Journal,2015,82(5):794-805.
[27] Mitea C,Kooy-Winkelaar Y,Van Veelen P,et al.Fine specificity of monoclonal antibodies against celiac diseaseinducing peptides in the gluteome[J].American Journal of Clinical Nutrition,2008,88(4):1057-1066.
[28] Londono D M,Van’t Westende W P C,Goryunova S,et al.Avenin diversity analysis of the genus Avena(oat).Relevance for people with celiac disease[J].Journal of Cereal Science,2013,58(1):170-177.
[29] Katagiri M,Masuda T,Tani F,et al.Expression and Development of Wheat Proteins during Maturation of Wheat Kernel and the Rheological Properties of Dough Prepared from the Flour of Mature and Immature Wheat[J].Food Science and Technology Research,2011,17(2):111-120.
[30] Kawaura K,Mochida K,Ogihara Y.Expression profile of two storage-protein gene families in hexaploid wheat revealed by large-scale analysis of expressed sequence tags[J].Plant Physiology,2005,139(4):1870-1880.
[31] Shewry P R,Mitchell R a C,Tosi P,et al.An integrated study of grain development of wheat(cv.Hereward)[J].Journal of Cereal Science,2012,56(1):21-30.
[32] Lai K T,Duran C,Berkman P J,et al.Single nucleotide polymorphism discovery from wheat next-generation sequence data[J].Plant Biotechnology Journal,2012,10(6):743-749.
[33] Banu M,Ng D,Zheng L,et al.Rapid quantification of Escherichia coli in food and media using bacteriophage T7amplification and liquid chromatography-multiple reaction monitoring tandem mass spectrometry[J].Journal of Biotechnology,2014,192:50-58.
[34] Van Den Broeck H C,Cordewener J H G,Nessen M A,et al.Label free targeted detection and quantification of celiac disease immunogenic epitopes by mass spectrometry[J].Journal of Chromatography A,2015,1391:60-71.
[35] Abbatiello S E,Mani D R,Schilling B,et al.Design,Implementation and Multisite Evaluation of a System Suitability Protocol for the Quantitative Assessment of Instrument Performance in Liquid Chromatography-Multiple Reaction Monitoring-MS(LC-MRM-MS)[J].Molecular&Cellular Proteomics,2013,12(9):2623-2639.
[36] Sealey-Voyksner J A,Khosla C,Voyksner R D,et al.Novel aspects of quantitation of immunogenic wheat gluten peptides by liquid chromatography-mass spectrometry/mass spectrometry[J].Journal of Chromatography A,2010,1217(25):4167-4183.
[37] Prandi B,Bencivenni M,Faccini A,et al.Composition of peptide mixtures derived from simulated gastrointestinal digestion of prolamins from different wheat varieties[J].Journal of Cereal Science,2012,56(2):223-231.
[38] Felber J,Stallmach A,Schuppan D.Not Celiac Disease,Non-Wheat Allergy,Wheat Sensitivity Comment[J].Zeitschrift Fur Gastroenterologie,2015,53(4):342-343.
[39] Salentijn E M J,Goryunova S V,Bas N,et al.Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci[J].BMC Genomics,2009,10.
[40] Elli L,Branchi F,Tomba C,et al.Diagnosis of gluten related disorders:Celiac disease,wheat allergy and non-celiac gluten sensitivity[J].World Journal of Gastroenterology,2015,21(23):7110-7119.
[41] Pietzak M.Celiac Disease,Wheat Allergy,and Gluten Sensitivity:When Gluten Free Is Not a Fad[J].Journal of Parenteral and Enteral Nutrition,2012,36:68s-75s.
计量
- 文章访问数: 157
- HTML全文浏览量: 19
- PDF下载量: 199
下载:
