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中国精品科技期刊2020

显微镜与实时荧光PCR检测方法在马蹄淀粉鉴定中的应用

陈展册, 杜智欣, 高佳, 刘军义, 罗兆飞

陈展册, 杜智欣, 高佳, 刘军义, 罗兆飞. 显微镜与实时荧光PCR检测方法在马蹄淀粉鉴定中的应用[J]. 食品工业科技, 2016, (20): 90-95. DOI: 10.13386/j.issn1002-0306.2016.20.009
引用本文: 陈展册, 杜智欣, 高佳, 刘军义, 罗兆飞. 显微镜与实时荧光PCR检测方法在马蹄淀粉鉴定中的应用[J]. 食品工业科技, 2016, (20): 90-95. DOI: 10.13386/j.issn1002-0306.2016.20.009
CHEN Zhan-ce, DU Zhi-xin, GAO Jia, LIU Jun-yi, LUO Zhao-fei. Application of the detection method by ligh microscopy and RT-PCR in authentication of water chestnut starch[J]. Science and Technology of Food Industry, 2016, (20): 90-95. DOI: 10.13386/j.issn1002-0306.2016.20.009
Citation: CHEN Zhan-ce, DU Zhi-xin, GAO Jia, LIU Jun-yi, LUO Zhao-fei. Application of the detection method by ligh microscopy and RT-PCR in authentication of water chestnut starch[J]. Science and Technology of Food Industry, 2016, (20): 90-95. DOI: 10.13386/j.issn1002-0306.2016.20.009

显微镜与实时荧光PCR检测方法在马蹄淀粉鉴定中的应用

基金项目: 

广西科技基础条件平台建设项目(12-112-01A);

详细信息
    作者简介:

    陈展册(1984-),女,硕士,农艺师,研究方向:植物检疫,E-mail:chenzhance@163.com。;

    罗兆飞(1978-),男,本科,高级兽医师,研究方向:食品安全,E-mail:luozf@gxicq.gov.cn。;

  • 中图分类号: TS237

Application of the detection method by ligh microscopy and RT-PCR in authentication of water chestnut starch

  • 摘要: 采用光学生物显微镜观测结合实时荧光PCR检测方法对马蹄淀粉中掺杂行为进行定性鉴别,以期为鉴别淀粉掺杂成分提供更客观、更准确的检测手段。结果显示:32个随意掺杂的马蹄淀粉样品中8个是纯马蹄淀粉,5个是纯木薯淀粉,1个是纯玉米淀粉,3个是纯马铃薯淀粉,3个是马蹄淀粉和马铃薯淀粉的混合物,1个是马蹄淀粉和木薯淀粉的混合物,1个是马蹄淀粉和玉米淀粉的混合物,5个是木薯和玉米淀粉的混合物,3个是木薯淀粉和马铃薯淀粉的混合物,2个是木薯淀粉、玉米淀粉、马铃薯淀粉的混合物。32个样品显微镜镜检结果和PCR检测结果基本吻合。两种方法可为国内淀粉市场质量监督提供有效的检测方法。 
    Abstract: The granular adulterated properties of water chestnut starch were investigated experimentally by using the modem analytical techniques such as light microscopy and RT- PCR assay. The results showed that eight commercialized samples were pure water chestnut starchs,five in 32 samples were pure tapioca starchs,one was pure corn starch,three were pure potato starchs,three were mixture of water chestnut starch and potato starch,one was mixture of water chestnut starch and tapioca starch,one was mixture of water chestnut starch and corn starch,five were mixture of tapioca starch and corn starch,three were mixture of tapioca starch and potato starch,two were mixture of tapioca starch,corn starch and potato starch.The results using microscopy was consistent with that of RT- PCR assay.Two kinds of detection methods could be used as product quality supervision of domestic starch market.
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  • 收稿日期:  2016-03-29

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