Effects of 1-methylcyclopropene and ethephon treatment on physiological changes and reactive oxygen metabolism of postharvest‘Golden Delicious' apple fruit during storage at ambient temperature
-
摘要: 以‘金冠’苹果为试材,研究1μL/L 1-甲基环丙烯(1-MCP)和0.2 mmol/L乙烯利处理对其采后常温贮藏过程中生理变化及活性氧相关代谢的影响。结果表明:与对照相比,1-MCP处理有效延缓果实软化、可滴定酸(TA)含量下降及可溶性固形物(TSS)含量上升,明显抑制叶绿素降解、呼吸强度及乙烯生成,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性,减缓O-2·产生速率、H2O2及丙二醛(MDA)的积累;乙烯利处理主要在前期促进果实软化和TA含量下降,促进叶绿素降解及乙烯生成,使呼吸高峰提前,对上述活性氧相关代谢有相反效果,但对TSS无显著影响。结论:1-MCP处理保持了采后苹果果实的常温贮藏品质,而乙烯利处理则相反,苹果果实采后贮藏过程中活性氧的代谢与乙烯有关。Abstract: Effects of 1 μL/L 1-methylcyclopropene ( 1-MCP) and 0.2 mmol/L ethephon on physiological changes and reactive oxygen metabolism of postharvest ‘Golden Delicious ' apples during storage at ambient temperature were investigated. The results showed that, compared with controls, 1-MCP treatment effectively delayed fruit softening, the decline of titratable acid ( TA) content and the increase in total soluble solids ( TSS) ; obviously inhibited chlorophyll degradation, respiration rate and ethylene production; increased the activity of superoxide dismutase ( SOD) , catalase ( CAT) and peroxidase ( POD) ; retarded superoxide anion ( O-2·) generation rate, the accumulation of H2O2 and malondialdehyde ( MDA) . Ethephon treatment mainly promoted fruit softening and the decline of TA content at the early storage period, accelerated the chlorophyll degradation and ethylene production, and also made the respiratory rate peak ahead of control. It had opposite effects on reactive oxygen metabolism mentioned above while had no significant effect on TSS content. In conclusion, 1-MCP treatment maintained the storage quality of postharvest apple fruit at ambient temperature, whereas ethephon treatment had opposite effects on it, the metabolism of reactive oxygen during postharvest storage of apple fruit was related to ethylene.
-
Keywords:
- apple /
- 1-methylcyclopropene /
- ethephon /
- postharvest physiology /
- reactive oxygen metabolism
-
[1] Barry C S, Giovannoni J J, et al.Ethylene and fruit ripening[J].Journal of Plant Growth Regulation, 2007, 26:143-159.
[2] Sisler E C, Serek M.Inhibitors of ethylene responses in plants at the receptor level:recent developments[J].Physiologia Plantarum, 1997, 100 (3) :577-582.
[3] 贾晓辉, 佟伟, 王文辉, 等.1-MCP、MAP对苹果冷藏期间品质及保鲜效果的影响[J].食品科学, 2011, 32 (8) :305-308. [4] 王风霞, 陆文文, 杨利侠, 等.低温及1-MCP处理对天水‘花牛’苹果贮藏品质与生理变化的影响分析[J].食品科学, 2014, 35 (22) :346-349. [5] 李江阔, 刘畅, 张鹏, 等.低温条件下不同时期1-MCP处理对金冠苹果生理和品质的影响[J].食品科学, 2015, 36 (18) :220-224. [6] 张佰清, 姜海峰, 魏宝东, 等.1-甲基环丙烯对岳冠苹果采后生理酶活性的影响[J].食品研究与开发, 2016, 37 (5) :137-141. [7] Liu R, Xu Y, Li B, et al.The role of 1-methylcyclopropene in lipid peroxidation, antioxidant enzyme activities, and ethylene biosynthesis in‘Laiyang’pear (Pyrus bretschneideri Rehd.) during fruit ripening[J].The Journal of Horticultural Science and Biotechnology, 2015, 90 (2) :210-216.
[8] Park YS, Im MH, Gorinstein S.Shelf life extension and antioxidant activity of‘Hayward’kiwifruit as a result of prestorage conditioning and 1-methylcyclopropene treatment[J].Journal of Food Science and Technology, 2015, 52 (5) :2711-2720.
[9] 张鲁斌, 贾志伟, 谷会.适宜1-MCP处理保持采后菠萝常温贮藏品质[J].农业工程学报, 2016, 32 (4) :290-296. [10] 饶景萍, 任小林.园艺产品贮运学[M].陕西:陕西人民出版社, 2003:37-38. [11] 郭香凤, 张国海, 史国安, 等.乙烯利对杏果实后熟的生理效应[J].河南农业大学学报, 2001, 35 (2) :122-124. [12] 郑诚乐, 王晓飞, 潘东明, 等.乙烯利处理对毛叶枣果实采后保护酶活性及膜透性的影响[J].江西农业大学学报, 2008, 30 (1) :36-39. [13] Singh R, Dwivedi UN.Effect of ethrel and1-methylcyclopropene (1-MCP) on antioxidants in mango (Mangifera indica var.Dashehari) during fruit ripening[J].Food Chemistry, 2008, 111 (4) :951-956.
[14] 胡位荣, 刘顺枝, 江月玲, 等.乙烯利处理对沙田柚采后果实品质及衰老的影响[J].果树学报, 2013, 30 (3) :458-464. [15] 葛永红, 李灿婴, 朱丹实, 等.采后柠檬酸处理对苹果青霉病的控制及其贮藏品质的影响[J].食品科学, 2014, 35 (22) :255-259. [16] 曹建康, 姜微波, 赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社, 2013:84-124. [17] Yang X, Song J, Campbell-Palmer L, et al.Effect of ethylene and 1-MCP on expression of genes involved in ethylene biosynthesis and perception during ripening of apple fruit[J].Postharvest Biology and Technology, 2013, 78 (4) :55-56.
[18] 张莹莹, 任小林, 王雷存, 等.苹果果实发育及成熟软化过程中LOX的活性特点[J].西北农业学报, 2010, 19 (9) :159-162. [19] 李富军, 翟衡, 杨洪强, 等.1-MCP对苹果果实贮藏期间乙烯合成代谢的影响[J].中国农业科学, 2004, 37 (5) :734-738. [20] 王云香, 张亚楠, 曲桂芹, 等.1-MCP处理对苹果采后常温贮藏品质的影响[J].食品科学, 2016, 37 (16) :280-285. [21] 郑丽静, 聂继云, 李明强, 等.苹果风味评价指标的筛选研究[J].中国农业科学, 2015, 48 (14) :2796-2805. [22] Hrtensteiner S, Krutler B.Chlorophyll breakdown in higher plants[J].Biochimica et Biophysica Acta, 2011, 1807 (8) :977-988.
[23] 田世平, 罗云波, 王桂禧.园艺产品采后生物学基础[M].北京:科学出版社, 2014:46-47. [24] 孙希生, 王文辉, 王志华, 等.1-MCP对苹果采后生理的影响[J].果树学报, 2003, 20 (1) :12-17. [25] 孙希生, 王志华, 辛广, 等.不同处理条件下1-MCP对金冠苹果呼吸强度和品质的影响[J].果树学报, 2004, 21 (2) :141-144. [26] 邓惠文, 赵磊, 许卫峰.不同浓度1-MCP对红富士苹果采后生理和贮藏效果的影响[J].陇东学院学报, 2015, 26 (5) :83-87. [27] 佟伟, 王文辉, 姜修成, 等.新型1-MCP缓释粉剂对新红星苹果贮藏效果研究[J].中国果树, 2015 (2) :20-23. [28] 程顺昌, 冷俊颖, 任小林, 等.不同环丙烯类乙烯抑制剂对苹果常温贮藏保鲜效果的影响[J].农业工程学报, 2012, 2 (2) :269-273. [29] 李志强, 乔玉山, 徐长宝, 等.不同后熟期菊水梨果实对外源乙烯和1-MCP处理的生理响应[J].西北植物学报, 2008, 28 (9) :1862-1867. [30] 魏建梅, 刘长江, 朱向秋, 等.苹果果实发育成熟软化过程中脂氧合酶活性变化及采后乙烯调控[J].河北农业科学, 2008, 12 (7) :27-30. [31] Whale S K, Singh Z, Behboudian M H, et al.Fruit quality in‘Cripp’s Pink’apple, especially colour, as affected by preharvest sprays of aminoethoxyvinylglycine and ethephon[J].Scientia Horticulturae, 2008, 115 (4) :342-351.
[32] Bower J H, Biasi W V, Mitcham E J.Effect of ethylene in the storage environment on quality of‘Bartlett pears’[J].Postharvest Biology and Technology, 2003, 28 (3) :371-379.
[33] Pranamomkith T, East A, Heyes J.Influence of exogenous ethylene during refrigerated storage on storability and quality of Actinidia chinensis (cv.Hort16A) [J].Postharvest Biology and Technology, 2012, 64 (1) :1-8.
[34] 魏颖超.ABA与乙烯对‘泰山早霞’苹果果实成熟的影响[D].山东农业大学, 2013:22-23. [35] 李泽珍, 狄建兵, 张杰.乙烯利处理对猕猴桃品质的影响[J].农产品加工, 2014, 6 (356) :8-12. [36] 胡花丽, 梁丽松, 王贵禧, 等.外源乙烯对CA贮藏桃果实品质变化的影响[J].食品科学, 2008, 29 (1) :338-342. [37] 贾晓辉, 王文辉, 佟伟, 等.乙烯对阿巴特梨后熟过程中生理效应的影响[J].吉林农业大学学报, 2009, 31 (4) :407-410. [38] Mittler R.Oxidative stress, antioxidants and stress tolerance[J].Trends in Plant Science, 2002, 7 (9) :405e410.
[39] Hong K Q, He Q G, Xu H B, et al.Effects of 1-MCP on oxidative parameters and quality in‘Pearl’guava (Psidium guajava L.) fruit[J].The Journal of Horticultural Science and Biotechnology, 2013, 88 (2) :117-122.
[40] 王晓飞, 杨艳青, 任小林, 等.1-MCP对‘粉红女士’苹果果实采后生理及其品质的影响[J].食品科学, 2014, 35 (18) :219-223.
计量
- 文章访问数: 219
- HTML全文浏览量: 35
- PDF下载量: 573