Preparation and characterization of carboxymethylatedlevan modified with collagen peptides
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摘要: 为考察羧甲基果聚糖及其衍生物在功能性食品、生物医学及化妆品方面的应用可行性,通过羧基与氨基的交联反应制备了胶原肽修饰的羧甲基果聚糖,并测试了产物的抗氧化、吸湿保湿活性以及其对人皮角质Ha Ca T细胞生长增殖的影响。发现羧甲基果聚糖经胶原肽修饰后抗氧化性明显提高,25 mg/m L时的ABTS+·清除率为62.31%,比修饰前提高了6.11倍。尽管胶原肽的引入会对吸湿保湿性造成影响,修饰后的羧甲基果聚糖仍然保留良好的吸湿保湿性能。在相对湿度43%和81%中放置72 h的吸湿率分别为11.96%和21.54%,在干燥硅胶环境中放置72 h的保湿率为60.48%。此外,修饰产物在01 mg/m L的浓度范围内无细胞毒性,可以促进Ha Ca T细胞增殖。Ha Ca T细胞在0.5 mg/m L修饰产物刺激下的存活率为124.2%。胶原肽修饰能提高羧甲基果聚糖的生物活性,拓展其在功能性食品、医药制品和化妆品上的应用潜力。Abstract: To investigate the possible application of carboxymethylatedlevan and its derivative on functional food, cosmetics and medicine, collagen peptides were used to modify carboxymethylated levan through the cross-linking reaction between carboxyl group and amino group.Moreover, the antioxidant activity and the moisture absorption and retention capacities of the modified product, as well as its effect on the viability of human skin keratinocyte cells ( Ha Ca T cells) were evaluated. After modified withcollagen peptides, the antioxidant activity of carboxymethylatedlevan was obviously improved. The scavenging rate of the product on ABTS+·was 6.11 times higher than that before modification, with the scavenging rate of 62.31% at 25 mg/m L.Though collagen peptides had some negative effect on the moisture absorption and retention capacities of the product, its modified carboxymethylatedlevan still had good moisture absorption and retention capacities.The moisture-absorbing rate of the product under the relative humidity of 43% and 81% was 11.96% and 21.54% at 72 h, respectively. The moisture retention capacity after desiccation in the silica gel chamber for 72 h was 60.48%. Furthermore, carboxymethylatedlevan modified with collagen peptides showed no cytotoxicity in the concentration of 0 ~ 1 mg/m L and could promote the proliferation of Ha Ca T cells.Under the stimulation of the modified product at 0.5 mg/m L, the cell viability of Ha Ca T cells reached 124.2%.The results showed that the modification with collagen peptides could improve the bioactivity of carboxymethylatedlevan, and extend its potential application on functional food, medicine and cosmetics.
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