Preparation of high-purity Nisin and the optimization of industrial scale production conditions
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摘要: 研究了一种制备高纯度Nisin的方法。该方法采用弱酸性离子交换树脂D113对Nisin浓缩液进行静态吸附和动态解吸,然后将解吸液进行盐析、抽滤、烘干、粉碎即得高纯度Nisin。结果表明p H为6,吸附时间为90 min,解吸剂为1 mol/L、V盐酸/V乙醇=1.25的盐酸乙醇溶液,解吸速率为1 BV/h,盐析所用氯化钠浓度为23%为最佳条件,获得的Nisin效价可达34000 IU/mg,收率达到67.45%,具有较好的工业应用前景。Abstract: In this study, a new method of preparation high-purity Nisin preparation was researched.The method adopted static adsorption and dynamic desorption of Nisin concentrated solution at a weak acidic ion exchange resin D113.The subsequent production process included salting out, suction filtration, drying and crushing to obtain high-purity Nisin. The results showed that the optimum condition was p H6, adsorption time 90 min, CDesorption agent= 1 mol/L, VHCl/VEt OHl= 1.25, desorption rate1 BV/h, CNa Cl= 23% in salting out.The potency of Nisin attained through this procedure was 34000 IU/mg, total yield reached67.45%.The method has bright prospect in industrial application.
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Keywords:
- high-purity /
- Nisin /
- ion exchange
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