Synergy inhibition effect of complex lactic acid bacteria derived fish on Vibrio harveyi
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摘要: 目的:优化抗哈维氏弧菌鱼源复合乳酸菌的组合。方法:应用牛津杯琼脂扩散法获得无交叉拮抗作用的乳酸菌菌株,利用正交实验优化对哈维氏弧菌具有较强协同抑制作用的乳酸菌组合。结果:获得4株对哈维氏弧菌有抑制效果且无交叉拮抗作用的乳酸菌,优化出菌株清酒乳杆菌YP4-5、清酒乳杆菌YP1-6和植物乳杆菌DY4-2组合以2∶3∶3的比例混合培养,复合发酵液在培养24 h时对哈维氏弧菌的抑菌直径为25.87 mm,分别比YP4-5、YP1-6和DY4-2单菌株的抑菌直径提升27.06%、37.10%和36.73%,表明三株乳酸菌具有协同作用。结论:植物乳杆菌DY4-2、清酒乳杆菌YP4-5和清酒乳杆菌YP1-6具有协同抑制哈维氏弧菌作用,三者以2∶3∶3组合对哈维氏弧菌的抑菌作用较强,可作为水产品养殖过程中控制哈维氏弧菌的乳酸菌复合制剂。Abstract: Objective: The aims of this study were to optimize an efficient combination of lactic acid bacteria (LAB) with strong antagonistic activity against Vibrio harveyi from the marine fish intestine.Methods: LAB with no cross antagonism were obtained using the method of Oxford cup agar diffusion. Complex LAB with strong synergy inhibition activity against V. harveyi was optimized by orthogonal experiment.Results: Lactic acid bacteria were obtained from the marine fish intestine, which had strong inhibition activity against V.harveyi and had no cross antagonism.The bacterial suspension of strain YP4-5, YP1-6 and DY4-2combined with the ratio of 2 ∶ 3 ∶ 3 reached the maximum zone of inhibitory diameter and reached 25.87 mm at 24 h, which increased 27.06%, 37.10% and 36.73% than that strain YP4-5, YP1-6, DY4-2 respectively.It indicated that the three strains LAB had the synergetic effects.Conclusion: Lb.plantarum DY4-2 and Lb. sake YP4-5, Lb. sake YP1-6 had synergetic effects.against V.harveyi, which combined with the ratio of 2 ∶ 3 ∶ 3 had strong antagonistic activity against V. harveyi. Therefore, the complex LAB can be used as the composite microbial agent for the control of V.harveyi in aquaculture.
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Keywords:
- lactic acid bacteria derived fish /
- complex /
- synergy inhibition /
- Vibrio harveyi
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