The stability of antioxidant peptides from squid and their anti-fatigue and anti-cancer activities
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摘要: 以鱿鱼抗氧化肽清除DPPH自由基和羟自由基的活性变化为指标,研究了温度、p H、金属离子、防腐剂和食品配料对鱿鱼抗氧化肽的抗氧化稳定性的影响,同时对鱿鱼抗氧化肽的抗疲劳和抗癌活性进行了初步研究。实验结果表明:鱿鱼抗氧化肽具有较强的耐热性,加热到100℃,仍能保持94%以上的活性;在酸性环境中活性保持较好,在碱性条件下活性丧失较快;蔗糖、Na Cl对鱿鱼抗氧化肽的稳定性影响不明显,而葡萄糖和苯甲酸钠会明显降低鱿鱼抗氧化肽活性（p<0.05）;Ca2+、Mg2+、Zn2+、Cu2+金属离子对鱿鱼抗氧化肽稳定性均有不同程度的影响,影响顺序为:Cu2+>Zn2+>Mg2+>Ca2+。鱿鱼抗氧化肽有较好的抗疲劳活性,与空白相比,抗氧化肽可以延长小鼠力竭游泳时间达到23%36%。鱿鱼抗氧化肽对胃癌细胞SGC-7901的增殖有明显的抑制作用,半数抑制率EC50为21.10 mg/m L。所以鱿鱼抗氧化肽是一种具有抗疲劳和抗癌活性且性质相对稳定的活性肽。Abstract: The change of scavenging activity on DPPH and hydroxyl free radicals of antioxidant peptides from squid was used as the index to study the effect of temperature, p H, different metal ions, preservatives and food ingredients on antioxidation stability of the peptides. At the same time, the anti-fatigue and anti-cancer activities of antioxidant peptides from squid were preliminarily studied.The results showed that antioxidant peptides from squid had strong heat-resistance, still maintaining more than 94% of the activity when heated to 100 ℃.The peptides maintained high activity under acidic conditions, but losed their activity under alkaline conditions.Surcrose and Na Cl had little effects on their antioxidation stability, but glucose and sodium benzoate lowered the oxidation stability of antioxidant peptides from squid ( p < 0.05) .Metal ions such as Ca2+, Mg2+, Zn2+and Cu2+affected the stability and the order was Cu2+> Zn2+> Mg2+> Ca2+. Antioxidant peptides from squid had better anti-fatigue activity.Compared with the blank, antioxidant peptides could prolong the exhaustive swimming time of mice to 23% 36%.Antioxidant peptides from squid had significant inhibition on proliferation of gastric cancer cell SGC-7901.The inhibition rate of EC50 was 21.10 mg/m L.Therefore, the antioxidant peptides from squid were a kind of active peptide with anti-fatigue, anti-cancer activity and stable property.
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Keywords:
- squid /
- antioxidant peptide /
- stability /
- anti-fatigue /
- anti-cancer
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[1] 林端权, 郭泽镔, 张怡, 等.海洋生物活性肽的研究进展[J].食品工业科技, 2016, 37 (18) :367-373. [2] L Najafian, AS Babji.A review of fish-derived antioxidant and antimicrobial peptides:Their production, assessment, and applications[J].Peptides, 2012, 33 (1) :178-185.
[3] Fukada Yoko, Mizutani Saki, Nomura Sarika, et al.Antioxidant activities of a peptide derived from chicken dark meat[J].Journal of Food Science and Technology, 2016, 53 (5) :476-481.
[4] Ohata, Motoko1, Uchida, et al.Antioxidant activity of fermented meat sauce and isolation of an associated antioxidant peptide[J].Food Chemistry, 2016, 194 (6) :1034-1039.
[5] 杜昕, 李诚, 肖岚, 等.酶解牦牛血发酵液制备抗氧化肽工艺优化[J].核农学报, 2017, 31 (2) :325-333. [6] Zhang Chong, Zhang Ningning, Li Zhiyu, et al.Stability of antioxidant peptides prepared from large yellow croaker (pseudosciaena crocea) [J].Current Topics in Nutraceutical Research, 2016, 14 (1) :37-47.
[7] Hye Lim Janga, Andrea M Liceagab, Kyung Young Yoona.Purification, characterisation and stability of an antioxidant peptide derived from sandfish (Arctoscopus japonicus) protein hydrolysates[J].Journal of Functional Foods, 2016, 20:433-442.
[8] Sah B N P, Vasiljevic T, Mc Kechnie S, et al.Effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel[J].Journal of Dairy Science, 2015, 98 (9) :5905-5916.
[9] 陈星星, 胡晓, 李来好, 等.抗疲劳肽的研究进展[J].食品工业科技, 2015, 36 (4) :365-369. [10] 吴非, 于胜男, 葛锡娟, 等.木瓜蛋白酶制备大豆抗癌活性肽的条件优化[J].食品科学, 2012, 33 (7) :148-152. [11] 胡小军, 江敏, 莫秋远, 等.鱿鱼肌肉蛋白肽的制备工艺优化及其抗氧化活性[J].食品工业科技, 2017, 38 (5) :191-195. [12] Zhu B W, Zhou D Y, Li T, et al.Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino) [J].Food Chemistry, 2010, 121 (3) :712-718.
[13] Motoko Ohata, Saeko Uchida, Lanxi Zhou, et al.Antioxidant activity of fermented meat sauce and isolation of an associated antioxidant peptide[J].Food Chemistry, 2016, 194 (6) :1034-1039.
[14] Sun Shuguo, Niu Huihui, Tao Yang, et al.Antioxidant and anti-fatigue activities of egg white peptides prepared by pepsin digestion[J].Journal of the Science of Food and Agriculture, 2014, 94 (15) :3195-3200.
[15] 葛锡娟, 许岩, 杨丽丽, 等.大豆抗癌肽的初步提取及抗癌活性[J].食品科学, 2011, 32 (16) :27-30. [16] Wang Zhujun, Zhang Xuewu.Inhibitory effects of small molecular peptides from Spirulina (Arthrospira) platensis on cancer cell growth[J].Food&Function, 2016, 7 (2) :781-788.
[17] 赵谋明, 何婷, 赵强忠, 等.蓝园鰺抗氧化肽抗氧化稳定性研究[J].食品科学, 2009, 30 (1) :128-130. [18] 王子怀, 胡晓, 李来好, 等.肽-金属离子螯合物的研究进展[J].食品工业科技, 2014, 35 (8) :359-362. [19] 胡晓, 吴静, 杨贤庆, 等.添加物和体外模拟胃肠道消化对鸢乌贼抗氧化肽稳定性的影响[J].食品与发酵工业, 2016, 42 (11) :91-96. [20] Amigo-Benavent M, del Castillo MD, Fogliano V.Are the major antioxidants derived from soy protein and fructooligosaccharides model systems colored aqueous soluble or insoluble compounds?[J].European Food Research and Technology, 2010, 231 (4) :545-553.
[21] 游丽君.泥鳅蛋白抗氧化肽的分离纯化及抗疲劳和抗癌功效研究[D].广州:华南理工大学, 2010:79-82.
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